Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged enzyme in Escherichia coli strain BL21 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bezafibrate | noncompetitive, IC50: 0.003 mM, recombinant enzyme | Homo sapiens | |
ciprofibrate | competitive, IC50: 0.00086 mM, recombinant enzyme | Homo sapiens | |
Clofibric acid | noncompetitive, IC50: 0.0012 mM, recombinant enzyme | Homo sapiens | |
fenofibrate | competitive, IC50: 0.00056 mM, recombinant enzyme | Homo sapiens | |
gemfibrozil | noncompetitive, IC50: 0.0065 mM, recombinant enzyme | Homo sapiens | |
sorbinil | competitive, IC50: 0.0021 mM, recombinant enzyme | Homo sapiens | |
zopolrestat | competitive, IC50: 0.00006 mM, recombinant enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
0.092 | - |
DL-glyceraldehyde | pH 6.2, 25°C, recombinant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography, removal of the His-tag by thrombin, and anion exchange chromatography to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + oxidized 3-acetylpyridine adenine dinucleotide + H+ | - |
Homo sapiens | benzaldehyde + reduced 3-acetylpyridine adenine dinucleotide | - |
r | |
DL-glyceraldehyde + NADPH + H+ | - |
Homo sapiens | glycerol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
aldose reductase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
DL-glyceraldehyde | pH 6.2, 25°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH preference depends on the substrate | Homo sapiens |
6.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, pH dependence of inhibition, overview | Homo sapiens | |
0.000043 | - |
zopolrestat | pH 6.2, 25°C, recombinant enzyme | Homo sapiens | |
0.00045 | - |
sorbinil | pH 6.2, 25°C, recombinant enzyme | Homo sapiens | |
0.00056 | - |
fenofibrat | pH 6.2, 25°C, recombinant enzyme | Homo sapiens | |
0.00088 | - |
ciprofibrate | pH 6.2, 25°C, recombinant enzyme | Homo sapiens | |
0.0011 | 0.0026 | bezafibrate | pH 6.2, 25°C, recombinant enzyme | Homo sapiens | |
0.0012 | 0.0037 | gemfibrozil | pH 6.2, 25°C, recombinant enzyme | Homo sapiens | |
0.00124 | - |
Clofibric acid | pH 6.2, 25°C, recombinant enzyme | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00006 | - |
competitive, IC50: 0.00006 mM, recombinant enzyme | Homo sapiens | zopolrestat | |
0.00056 | - |
competitive, IC50: 0.00056 mM, recombinant enzyme | Homo sapiens | fenofibrate | |
0.00086 | - |
competitive, IC50: 0.00086 mM, recombinant enzyme | Homo sapiens | ciprofibrate | |
0.0012 | - |
noncompetitive, IC50: 0.0012 mM, recombinant enzyme | Homo sapiens | Clofibric acid | |
0.0021 | - |
competitive, IC50: 0.0021 mM, recombinant enzyme | Homo sapiens | sorbinil | |
0.003 | - |
noncompetitive, IC50: 0.003 mM, recombinant enzyme | Homo sapiens | bezafibrate | |
0.0065 | - |
noncompetitive, IC50: 0.0065 mM, recombinant enzyme | Homo sapiens | gemfibrozil |