Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 10-15% increased activity | Yamadazyma tenuis | |
Na2SO4 | - |
Yamadazyma tenuis | |
Tween | Tween-20, Tween-80, Tween-100, each 0.1% (w/v) | Yamadazyma tenuis |
General Stability | Organism |
---|---|
25°C, 0.3 M phosphate or Tris, pH 6.0, more than 2 months | Yamadazyma tenuis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Yamadazyma tenuis | |
ATP | - |
Yamadazyma tenuis | |
dithiothreitol | - |
Yamadazyma tenuis | |
EDTA | - |
Yamadazyma tenuis | |
ionic detergents | - |
Yamadazyma tenuis | |
Mn2+ | - |
Yamadazyma tenuis | |
NADP+ | - |
Yamadazyma tenuis | |
pyridoxal 5'-phosphate | - |
Yamadazyma tenuis | |
Zn2+ | - |
Yamadazyma tenuis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | brief investigation of steady state kinetic | Yamadazyma tenuis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Yamadazyma tenuis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yamadazyma tenuis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Yamadazyma tenuis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alditol + NAD(P)+ = aldose + NAD(P)H + H+ | reaction mechanism | Yamadazyma tenuis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
- |
Yamadazyma tenuis |
28.64 | - |
purified enzyme | Yamadazyma tenuis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alditol + NAD(P)+ | 10% oxidation activity compared to reduction reaction | Yamadazyma tenuis | aldose + NAD(P)H | - |
r | |
aldose + NAD(P)H | wide specificity | Yamadazyma tenuis | alditol + NAD(P)+ | - |
r | |
D-xylose + NAD(P)H | - |
Yamadazyma tenuis | xylitol + NAD(P)+ | - |
r | |
pentose + NADPH | - |
Yamadazyma tenuis | pentitol + NADP+ | - |
r | |
xylitol + NAD(P)+ | - |
Yamadazyma tenuis | D-xylose + NAD(P)H + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Yamadazyma tenuis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Yamadazyma tenuis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Yamadazyma tenuis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | after 48 h incubation activity decreases | Yamadazyma tenuis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of D-xylose, NADH | Yamadazyma tenuis |
8.9 | - |
oxidation of xylitol, NAD+ | Yamadazyma tenuis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | below pH 5.0 and above pH 8.0 inactivation at 25°C within 3-6 days | Yamadazyma tenuis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Yamadazyma tenuis | |
NADP+ | 2.2fold higher activity than with NAD+ | Yamadazyma tenuis | |
NADPH | 5.3fold higher activity than with NAD(H) | Yamadazyma tenuis |