Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Escherichia coli | |
AMP | - |
Escherichia coli | |
ATP | - |
Escherichia coli | |
GDP | - |
Escherichia coli | |
GMP | - |
Escherichia coli | |
GTP | - |
Escherichia coli | |
Mg2+ | inhibits K+ activation | Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli | |
xanthosine 5'-phosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activation | Escherichia coli | |
Na+ | no effect | Escherichia coli | |
NH4+ | activation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
various molecular species | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
derepressed mutant | - |
Purification (Comment) | Organism |
---|---|
derepressed mutant | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-17°C, 24 h, 10% loss of activity | Escherichia coli |
4°C, 24 h, 13% loss of activity | Escherichia coli |
frozen in 5 mM mercaptoethanol, 3 months, 50% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine 5'-phosphate + NAD+ + H2O | - |
Escherichia coli | xanthosine 5'-phosphate + NADH | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
13% loss of activity within 1 day | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetylpyridine-NAD+ | 60% as effective as NAD+ | Escherichia coli | |
NAD+ | - |
Escherichia coli | |
NADP+ | not | Escherichia coli | |
thionicotinamide-NAD+ | 5% as effective as NAD+ | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition by purine nucleotides | Escherichia coli |