General Stability | Organism |
---|---|
NAD+ increases stability | Pseudomonas syringae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 1 mM | Pseudomonas syringae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
D-galacturonate | - |
Pseudomonas syringae | |
0.15 | - |
D-glucuronate | - |
Pseudomonas syringae | |
5.3 | - |
NAD+ | with 0.67 mM D-galacturonate | Pseudomonas syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NAD+ + H2O | Pseudomonas syringae | 50% of the activity compared to D-glucoronate | D-galactarate + NADH + H+ | - |
ir | |
D-glucuronate + NAD+ + H2O | Pseudomonas syringae | strong substrate specificity, best substrate | D-glucaric acid + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Pseudomonas syringae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas syringae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Pseudomonas syringae |
Storage Stability | Organism |
---|---|
-10°C, 50 mM Tris-HCl, pH 7.2, 2.9 mM NAD+, less then 10% loss of activity up to 6 months | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NAD+ + H2O | 50% of the activity compared to D-glucoronate | Pseudomonas syringae | D-galactarate + NADH + H+ | - |
ir | |
D-glucuronate + NAD+ + H2O | strong substrate specificity, best substrate | Pseudomonas syringae | D-glucaric acid + NADH + H+ | - |
ir |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
stable | Pseudomonas syringae |
30 | 35 | loss of activity after 30 min | Pseudomonas syringae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.4 | - |
Pseudomonas syringae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
50% remaining activity | Pseudomonas syringae |
9.4 | - |
50% remaining activity | Pseudomonas syringae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can not be replaced by NADP+ | Pseudomonas syringae |