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Literature summary for 1.1.1.200 extracted from
- Identification and functional characterization of sorbitol-6-phosphate dehydrogenase protein from rice and structural elucidation by in silico approach (2014), Planta, 240, 223-238.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.21 | - |
D-sorbitol 6-phosphate | at pH 8.0 and 25°C | Oryza sativa |  |
| 15.9 | - |
D-glucose 6-phosphate | at pH 8.0 and 25°C | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
x * 39000, SDS-PAGE | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Oryza sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.477 | - |
with D-sorbitol 6-phosphate as substrate, at pH 8.0 and 25°C | Oryza sativa |
| 0.603 | - |
with D-glucose 6-phosphate as substrate, at pH 8.0 and 25°C | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADPH + H+ | - |
Oryza sativa | D-sorbitol 6-phosphate + NADP+ | - |
r | |
| D-sorbitol 6-phosphate + NADP+ | - |
Oryza sativa | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
| additional information | no activity against mannose 6-phosphate | Oryza sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S6PDH | - |
Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Oryza sativa | |
| NADPH | - |
Oryza sativa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | sorbitol-6-phosphate dehydrogenase is a key enzyme for sorbitol synthesis and plays an important role in the alleviation of salinity stress in plants | Oryza sativa |
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Release 2023.1 (January 2023)
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