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Literature summary for 1.1.1.200 extracted from

  • Figueroa, C.M.; Iglesias, A.A.
    Aldose-6-phosphate reductase from apple leaves: Importance of the quaternary structure for enzyme activity (2010), Biochimie, 92, 81-88.
    View publication on PubMed
Search for more literature for 1.1.1.200

Cloned(Commentary)

Cloned (Comment) Organism
amplified gene cloned into the pGEM-T Easy vector, heterologous expression with a His-tag alternatively placed in the N- or C-terminus in Escherichia coli BL21 Star (DE3) cells Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Malus domestica
KCl
-
 Malus domestica
additional information no significant differences in activity when ATP, ADP, AMP, ADP-glucose, UTP, UDP-glucose, inorganic phosphate or diphosphate (5 mM each), 10% (v/v) glycerol, 500 mM glucitol or 20% (w/v) sucrose are added to the enzyme assay mixture Malus domestica
NaCl 200 mM cause about 65% inhibition of the enzyme activity Malus domestica
NH4Cl
-
 Malus domestica

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble level of soluble recombinant enzyme is much higher in the N-term tagged protein than in the C-term tagged one. In the C-term tagged one the majority of the protein is in the insoluble form Malus domestica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34900
-
 sequence analysis, A6PRase Malus domestica
39000
-
 gel filtration, C-term tagged enzyme Malus domestica
68000
-
 gel filtration, N-term tagged enzyme Malus domestica

Organism

Organism UniProt Comment Textmining
Malus domestica P28475
-
-

Purification (Commentary)

Purification (Comment) Organism
one-step protein purification by immobilized metal affinity chromatography, N-term tagged protein 95% pure, C-term tagged one 75% pure Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Malus domestica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
 C-term tagged enzyme, purified enzyme Malus domestica
0.136
-
 N-term tagged enzyme, crude extract Malus domestica
1.25
-
 N-term tagged enzyme, 9.2fold purified Malus domestica

Storage Stability

Storage Stability Organism
-80°C, 25 mM Tris-HCl buffer, pH 8.0, 300 mM NaCl, 1 mM beta-mercaptoethanol, 10% (v/v) glycerol, 1 mM EDTA, 0.5 mM DTT, 6 months Malus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucitol-6-phosphate
-
 Malus domestica D-glucose 6-phosphate + NADPH + H+
-
 r
D-glucose 6-phosphate + NADPH + H+
-
 Malus domestica D-glucitol-6-phosphate + NADP+
-
 r
additional information both, the N- and the C-term tagged enzymes exhibit similar affinity toward substrates Malus domestica ?
-
 ?

Subunits

Subunits Comment Organism
dimer gel filtration, N-term tagged enzyme Malus domestica
monomer gel filtration, C-term tagged enzyme Malus domestica

Synonyms

Synonyms Comment Organism
A6PRase
-
 Malus domestica
aldose-6-phosphate reductase
-
 Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0011
-
 D-glucitol-6-phosphate C-term tagged enzyme Malus domestica
0.009
-
 D-glucose 6-phosphate C-term tagged enzyme Malus domestica
0.222
-
 D-glucitol-6-phosphate N-term tagged enzyme Malus domestica
0.7
-
 D-glucose 6-phosphate N-term tagged enzyme Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
6 10 N- and the C-term tagged enzymes. Both recombinant enzymes similarly show optimal activity in a broad pH range in the direction of D-glucose 6-phosphate reduction, and a narrow range (pH 9.0-10.0) in the opposite oxidative direction Malus domestica