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Literature summary for 1.1.1.2 extracted from

  • Elleuche, S.; Fodor, K.; von der Heyde, A.; Klippel, B.; Wilmanns, M.; Antranikian, G.
    Group III alcohol dehydrogenase from Pectobacterium atrosepticum: insights into enzymatic activity and organization of the metal ion-containing region (2014), Appl. Microbiol. Biotechnol., 98, 4041-4051.
    View publication on PubMed
Search for more literature for 1.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pectobacterium atrosepticum

Protein Variants

Protein Variants Comment Organism
D194A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
H198A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
H267A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
H281A mutation in putative metal-coordinating residue, almost complete loss of activity Pectobacterium atrosepticum
H363A 107% of wild-type activity Pectobacterium atrosepticum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.1 mM, 38% residual activity. Activity is completely restored by the addition of 1 mM Co2+ and to 37% by addition of Ni2+, respectively Pectobacterium atrosepticum
NADPH strong inhibition at high NADPH concentrations, assay below 0.8 mM Pectobacterium atrosepticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NADPH pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
0.4
-
 pentanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
6.8
-
 Butanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
 Pectobacterium atrosepticum
42100
-
-
 Pectobacterium atrosepticum
71000
-
 gel filtration Pectobacterium atrosepticum

Organism

Organism UniProt Comment Textmining
Pectobacterium atrosepticum U6CL97
-
-
Pectobacterium atrosepticum DSM 18077 U6CL97
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanal + NADPH + H+
-
 Pectobacterium atrosepticum butan-1-ol + NADP+
-
 ?
butanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM18077 butan-1-ol + NADP+
-
 ?
butanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM 18077 butan-1-ol + NADP+
-
 ?
additional information enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor Pectobacterium atrosepticum ?
-
 ?
additional information enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor Pectobacterium atrosepticum DSM18077 ?
-
 ?
additional information enzyme does not catalyze the oxidation of alcoholic substrates butanol, propanol, ethanol, methanol, glycerol, 1,3-propanediol, and 1,2-propandiol with NAD(P)+ as cofactor Pectobacterium atrosepticum DSM 18077 ?
-
 ?
pentanal + NADPH + H+
-
 Pectobacterium atrosepticum pentan-1-ol + NADP+
-
 ?
pentanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM18077 pentan-1-ol + NADP+
-
 ?
pentanal + NADPH + H+
-
 Pectobacterium atrosepticum DSM 18077 pentan-1-ol + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42100, calculated, 2 * 42000, SDS-PAGE Pectobacterium atrosepticum

Synonyms

Synonyms Comment Organism
YqhD
-
 Pectobacterium atrosepticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pectobacterium atrosepticum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30 30-60% of maximum activity Pectobacterium atrosepticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 40 stable for at least 30 min Pectobacterium atrosepticum
50
-
 complete inactivation Pectobacterium atrosepticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.1
-
 Butanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
49.2
-
 pentanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pectobacterium atrosepticum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pectobacterium atrosepticum

pI Value

Organism Comment pI Value Maximum pI Value
Pectobacterium atrosepticum calculated
-
 5.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 Butanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum
123.1
-
 pentanal pH 7.0, temperature not specified in the publication Pectobacterium atrosepticum