Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
0.007 | - |
NADPH | native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
0.84 | - |
acetaldehyde | recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
1 | - |
acetaldehyde | native enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
9.7 | - |
ethanol | recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
9.7 | - |
NADP+ | recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
10.4 | - |
ethanol | native enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
10.4 | - |
NADP+ | native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 1.0 g-atom iron per subunit | Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Thermococcus sp. |
176000 | - |
recombinant enzyme, gel filtration | Thermococcus sp. |
179200 | - |
calculated from amino acid sequence | Thermococcus sp. |
180000 | - |
SDS-PAGE | Thermococcus sp. |
182000 | - |
native enzyme, gel filtration | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | C1IWT4 | - |
- |
Thermococcus sp. ES1 | C1IWT4 | - |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is sensitive to oxygen with a half-life upon exposure to air of about 4 min | Thermococcus sp. |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration | Thermococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
native enzyme from cell-free extract, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. |
6.8 | - |
recombinant enzyme from cell-free extract, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. |
52.8 | - |
recombinant enzyme after 7.8fold purification, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. |
57 | - |
native enzyme after 10.1fold purification, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NADP+ | the native enzyme shows 154.6% activity with 1-butanol compared to ethanol | Thermococcus sp. | butanal + NADPH + H+ | - |
? | |
1-butanol + NADP+ | the native enzyme shows 154.6% activity with 1-butanol compared to ethanol | Thermococcus sp. ES1 | butanal + NADPH + H+ | - |
? | |
1-heptanol + NADP+ | the native enzyme shows 94% activity with 1-heptanol compared to ethanol | Thermococcus sp. | heptanal + NADPH + H+ | - |
? | |
1-heptanol + NADP+ | the native enzyme shows 94% activity with 1-heptanol compared to ethanol | Thermococcus sp. ES1 | heptanal + NADPH + H+ | - |
? | |
1-octanol + NADP+ | the native enzyme shows 68.8% activity with 1-octanol compared to ethanol | Thermococcus sp. | octanal + NADPH + H+ | - |
? | |
1-octanol + NADP+ | the native enzyme shows 68.8% activity with 1-octanol compared to ethanol | Thermococcus sp. ES1 | octanal + NADPH + H+ | - |
? | |
1-pentanol + NADP+ | the native enzyme shows 184% activity with 1-pentanol compared to ethanol | Thermococcus sp. | pentanal + NADPH + H+ | - |
? | |
1-pentanol + NADP+ | the native enzyme shows 184% activity with 1-pentanol compared to ethanol | Thermococcus sp. ES1 | pentanal + NADPH + H+ | - |
? | |
1-propanol + NADP+ | the native enzyme shows 120.6% activity with 1-propanol compared to ethanol | Thermococcus sp. | propanal + NADPH + H+ | - |
? | |
2-phenylethanol + NADP+ | the native enzyme shows 40.2% activity with 2-phenylethanol compared to ethanol | Thermococcus sp. | 2-phenylacetaldehyde + NADPH + H+ | - |
? | |
ethanol + NADP+ | the native enzyme shows 100% activity with ethanol | Thermococcus sp. | acetaldehyde + NADPH + H+ | - |
? | |
ethanol + NADP+ | the native enzyme shows 100% activity with ethanol | Thermococcus sp. ES1 | acetaldehyde + NADPH + H+ | - |
? | |
hexyl alcohol + NADP+ | the native enzyme shows 137.8% activity with hexyl alcohol compared to ethanol | Thermococcus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 45000, SDS-PAGE | Thermococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Thermococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.9 | - |
acetaldehyde | recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
23.6 | - |
acetaldehyde | native enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
24 | - |
NADPH | native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
24 | - |
NADPH | recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
81 | - |
ethanol | recombinant enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
81 | - |
NADP+ | recombinant enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
84 | - |
ethanol | native enzyme, with 0.4 mM NADP+ as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. | |
84 | - |
NADP+ | native enzyme, with 90 mM ethanol as cosubstrate, in 100 mM EPPS buffer (pH 8.8) | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
for acetaldehyde reduction | Thermococcus sp. |
10.3 | 10.5 | for ethanol oxidation | Thermococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermococcus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermococcus sp. | calculated from amino acid sequence | - |
5.5 |