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Literature summary for 1.1.1.2 extracted from

  • Wahlen, B.D.; Oswald, W.S.; Seefeldt, L.C.; Barney, B.M.
    Purification, characterization, and potential bacterial wax production role of an NADPH-dependent fatty aldehyde reductase from Marinobacter aquaeolei VT8 (2009), Appl. Environ. Microbiol., 75, 2758-2764.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
industry possible uses of this enzyme in the production of wax esters Marinobacter nauticus VT8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain TB1 as a fusion protein with the maltose binding protein on the N-terminus Marinobacter nauticus VT8

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 24% Marinobacter nauticus VT8
beta-mercaptoethanol in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 25% Marinobacter nauticus VT8
diethyldithiocarbamate in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 50%, 0.25 mM inhibits by 39% Marinobacter nauticus VT8
dipyridyl in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 54%, 0.25 mM inhibits by 41% Marinobacter nauticus VT8
Dithionite in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 75%, 0.25 mM inhibits by 92% Marinobacter nauticus VT8
dithiothreitol in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 24% Marinobacter nauticus VT8
Dodecanol in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 46%, 0.25 mM inhibits by 45% Marinobacter nauticus VT8
EDTA in 100 mM MES buffer (pH 6.5) with 200 mM NADPH, 0.25 mM decanal, and 125 microg of FALDR fusion protein. 1 mM inhibits by 19% Marinobacter nauticus VT8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.177
-
cis-11-hexadecenal
-
Marinobacter nauticus VT8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
1 * 57000, SDS-PAGE Marinobacter nauticus VT8

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus VT8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity by amylose affinity chromatography Marinobacter nauticus VT8

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.085
-
highest specific activity for the reduction of decanal Marinobacter nauticus VT8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-11-hexadecenal + NADPH 87% relative activity Marinobacter nauticus VT8 cis-11-hexadecenol + NADP+
-
ir
decanal + NADPH no detectable catalysis of the reverse reaction, the oxidation of decanol by NADP+. 100% relative activity Marinobacter nauticus VT8 decanol + NADP+
-
ir
dodecanal + NADPH 55% relative activity Marinobacter nauticus VT8 dodecanol + NADP+
-
ir
additional information no activity with butanal, hexanal and benzaldehyde Marinobacter nauticus VT8 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE Marinobacter nauticus VT8

Synonyms

Synonyms Comment Organism
NADPH-dependent FALDR
-
Marinobacter nauticus VT8
NADPH-dependent fatty aldehyde reductase
-
Marinobacter nauticus VT8

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
Marinobacter nauticus VT8

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8
-
Marinobacter nauticus VT8

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Marinobacter nauticus VT8
NADPH
-
Marinobacter nauticus VT8