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Literature summary for 1.1.1.2 extracted from

  • Colrat, S.; Latche, A.; Guis, M.; Pech, J.C.; Bouzayen, M.; Fallot, J.; Roustan, J.P.
    Purification and characterization of a NADPH-dependent aldehyde reductase from mung bean that detoxifies eutypine, a toxin from Eutypa lata1 (1999), Plant Physiol., 119, 621-626.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.2

Application

Application Comment Organism
agriculture detoxification of eutypine toxin from Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
 m-nitrobenzaldehyde
-
 Vigna radiata
0.0033
-
 decanal
-
 Vigna radiata
0.0045
-
 4-pyridine carboxaldehyde
-
 Vigna radiata
0.0053
-
 4-nitrobenzaldehyde
-
 Vigna radiata
0.0054
-
 3-Fluorobenzaldehyde
-
 Vigna radiata
0.0063
-
 (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) eutypine Vigna radiata
0.015
-
 1-hexanal
-
 Vigna radiata
0.02
-
 m-methylbenzaldehyde
-
 Vigna radiata
0.033
-
 benzaldehyde
-
 Vigna radiata
0.04
-
 p-fluorobenzaldehyde
-
 Vigna radiata
0.041
-
 p-methylbenzaldehyde
-
 Vigna radiata
0.049
-
 2-Methoxybenzaldehyde
-
 Vigna radiata
0.06
-
 coniferylaldehyde
-
 Vigna radiata
0.066
-
 Salicylaldehyde
-
 Vigna radiata
0.087
-
 propionaldehyde
-
 Vigna radiata
0.099
-
 cinnamaldehyde
-
 Vigna radiata
0.125
-
 o-methylbenzaldehyde
-
 Vigna radiata
0.126
-
 sinapylaldehyde
-
 Vigna radiata
0.15
-
 p-Methoxybenzaldehyde
-
 Vigna radiata
0.207
-
 furaldehyde
-
 Vigna radiata
0.212
-
 m-methoxybenzaldehyde
-
 Vigna radiata
0.376
-
 o-nitrobenzaldehyde
-
 Vigna radiata
0.702
-
 o-fluorobenzaldehyde
-
 Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
-
 Vigna radiata
36000
-
 1 * 36000 Thermococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+ Vigna radiata eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+ eutypinol, non-toxic metabolite ir

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
-
-
Vigna radiata
-
 mung bean
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 891 nkat/mg Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+ eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera Vigna radiata (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+ eutypinol, non-toxic metabolite ir
2-methoxybenzaldehyde + NADPH + H+
-
 Vigna radiata 2-methoxybenzyl alcohol + NADP+
-
 ?
4-pyridinecarboxaldehyde + NADPH
-
 Vigna radiata 4-pyridinemethanol + NADP+
-
 ?
benzaldehyde + NADPH
-
 Vigna radiata benzyl alcohol + NADP+
-
 ?
coniferaldehyde + NADPH converted less efficiently than eutypine Vigna radiata coniferyl alcohol + NADP+
-
 ?
furaldehyde + NADPH
-
 Vigna radiata furfuryl alcohol + NADP+
-
 ?
hexanal + NADPH + H+
-
 Vigna radiata hexanol + NADP+
-
 ?
m-fluorobenzaldehyde + NADPH + H+
-
 Vigna radiata m-fluorobenzyl alcohol + NADP+
-
 ?
m-methoxybenzaldehyde + NADPH + H+
-
 Vigna radiata m-methoxybenzyl alcohol + NADP+
-
 ?
m-methylbenzaldehyde + NADPH
-
 Vigna radiata m-methylbenzyl alcohol + NADP+
-
 ?
m-nitrobenzaldehyde + NADPH
-
 Vigna radiata m-nitrobenzylalcohol + NADP+
-
 ?
additional information
-
 Thermococcus sp. ?
-
 ?
additional information dihydroquercitin and dihydromyricetin are no substrates Vigna radiata ?
-
 ?
n-decanal + NADPH + H+ converted less efficiently than eutypine Vigna radiata n-decanol + NADP+
-
 ?
o-fluorobenzaldehyde + NADPH + H+
-
 Vigna radiata o-fluorobenzyl alcohol + NADP+
-
 ?
o-methylbenzaldehyde + NADPH
-
 Vigna radiata o-methylbenzyl alcohol + NADP+
-
 ?
o-nitrobenzaldehyde + NADPH
-
 Vigna radiata o-nitrobenzylalcohol + NADP+
-
 ?
p-fluorobenzaldehyde + NADPH + H+
-
 Vigna radiata p-fluorobenzyl alcohol + NADP+
-
 ?
p-methoxybenzaldehyde + NADPH + H+
-
 Vigna radiata p-methoxybenzyl alcohol + NADP+
-
 ?
p-methylbenzaldehyde + NADPH
-
 Vigna radiata p-methylbenzyl alcohol + NADP+
-
 ?
p-nitrobenzaldehyde + NADPH
-
 Vigna radiata p-nitrobenzylalcohol + NADP+
-
 ?
propionaldehyde + NADPH
-
 Vigna radiata propanol + NADP+
-
 ?
salicylaldehyde + NADPH
-
 Vigna radiata 2-hydroxymethylphenol + NADP+
-
 ?
sinapaldehyde + NADPH + H+ converted less efficiently than eutypine Vigna radiata sinapyl alcohol + NADP+
-
 ?
trans-cinnamaldehyde + NADPH converted less efficiently than eutypine Vigna radiata cinnamyl alcohol + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Vigna radiata
monomer 1 * 36000 Thermococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.5
-
 Vigna radiata

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Vigna radiata
NADPH
-
 Thermococcus sp.