Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leucaena leucocephala |
Crystallization (Comment) | Organism |
---|---|
homology modeling based on structure of Arabidopsis thaliana isoform CAD5. The model reveals a trilobed structure. The two major lobes contribute to constitution of NADP+ binding, catalytic Zn2+ binding and substrate binding domains. The substrate binding pocket is protected by these two major lobes and is in close proximity of catalytic Zn2+ and NADP+ binding domains | Leucaena leucocephala |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0122 | - |
cinnamyl alcohol | pH 8.8, 37°C | Leucaena leucocephala | |
0.0181 | - |
coniferyl alcohol | pH 8.8, 37°C | Leucaena leucocephala | |
0.0238 | - |
sinapyl alcohol | pH 8.8, 37°C | Leucaena leucocephala |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39772 | - |
x * 39772, calculated, x * 40000, SDS-PAGE | Leucaena leucocephala |
40000 | - |
x * 39772, calculated, x * 40000, SDS-PAGE | Leucaena leucocephala |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | B5TR18 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
xylem | - |
Leucaena leucocephala | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamyl alcohol + NADP+ | - |
Leucaena leucocephala | cinnamyl aldehyde + NADPH + H+ | - |
? | |
coniferyl alcohol + NADP+ | - |
Leucaena leucocephala | coniferyl aldehyde + NADPH + H+ | - |
? | |
sinapyl alcohol + NADP+ | - |
Leucaena leucocephala | sinapyl aldehyde + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39772, calculated, x * 40000, SDS-PAGE | Leucaena leucocephala |
Organism | Comment | Expression |
---|---|---|
Leucaena leucocephala | presence of Zn2+ is essential for the expression of enzyme, as no expression is observed in absence of Zn2+ | additional information |