Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.195 extracted from

  • Pandey, B.; Pandey, V.P.; Dwivedi, U.N.
    Cloning, expression, functional validation and modeling of cinnamyl alcohol dehydrogenase isolated from xylem of Leucaena leucocephala (2011), Protein Expr. Purif., 79, 197-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leucaena leucocephala

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling based on structure of Arabidopsis thaliana isoform CAD5. The model reveals a trilobed structure. The two major lobes contribute to constitution of NADP+ binding, catalytic Zn2+ binding and substrate binding domains. The substrate binding pocket is protected by these two major lobes and is in close proximity of catalytic Zn2+ and NADP+ binding domains Leucaena leucocephala

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0122
-
cinnamyl alcohol pH 8.8, 37°C Leucaena leucocephala
0.0181
-
coniferyl alcohol pH 8.8, 37°C Leucaena leucocephala
0.0238
-
sinapyl alcohol pH 8.8, 37°C Leucaena leucocephala

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39772
-
x * 39772, calculated, x * 40000, SDS-PAGE Leucaena leucocephala
40000
-
x * 39772, calculated, x * 40000, SDS-PAGE Leucaena leucocephala

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala B5TR18
-
-

Source Tissue

Source Tissue Comment Organism Textmining
xylem
-
Leucaena leucocephala
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamyl alcohol + NADP+
-
Leucaena leucocephala cinnamyl aldehyde + NADPH + H+
-
?
coniferyl alcohol + NADP+
-
Leucaena leucocephala coniferyl aldehyde + NADPH + H+
-
?
sinapyl alcohol + NADP+
-
Leucaena leucocephala sinapyl aldehyde + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 39772, calculated, x * 40000, SDS-PAGE Leucaena leucocephala

Expression

Organism Comment Expression
Leucaena leucocephala presence of Zn2+ is essential for the expression of enzyme, as no expression is observed in absence of Zn2+ additional information