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Literature summary for 1.1.1.195 extracted from

  • Nishimura, M.; Kohno, K.; Nishimura, Y.; Inagaki, M.; Davies, J.
    Characterization of two isozymes of coniferyl alcohol dehydrogenase from Streptomyces sp. NL15-2K (2011), Biosci. Biotechnol. Biochem., 75, 1770-1777.
    View publication on PubMed
Search for more literature for 1.1.1.195

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 1 mM, 74% residual activity Streptomyces sp.
iodoacetic acid 1 mM, 56% residual activity Streptomyces sp.
N-bromosuccimide 1 mM, 56% residual activity Streptomyces sp.
Zn2+ 1 mM, 80% residual activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
0.17
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
0.5
-
 allyl alcohol pH 8.0, 30°C Streptomyces sp.
0.92
-
 cinnamaldehyde pH 8.0, 30°C Streptomyces sp.
0.96
-
 3-Phenyl-1-propanol pH 8.0, 30°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35782
-
 4 * 35782, MALDI-TOF Streptomyces sp.
143000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phenyl-1-propanol + NAD+
-
 Streptomyces sp. 3-phenylpropanal + NADH + H+
-
 r
allyl alcohol + NAD+
-
 Streptomyces sp. prop-2-enal + NADH + H+
-
 r
cinnamaldehyde + NADH + H+
-
 Streptomyces sp. cinnamyl alcohol + NAD+
-
 r
cinnamyl alcohol + NADP+
-
 Streptomyces sp. cinnamaldehyde + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 35782, MALDI-TOF Streptomyces sp.

Synonyms

Synonyms Comment Organism
CADH I
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.0, stable up to Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.2
-
 3-Phenyl-1-propanol pH 8.0, 30°C Streptomyces sp.
14.4
-
 cinnamaldehyde pH 8.0, 30°C Streptomyces sp.
18.1
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
19.9
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
32.9
-
 allyl alcohol pH 8.0, 30°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 75 h, more than 80% of initial activity Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
additional information NADPH is about 4000fold less effective than NADH Streptomyces sp.
NAD+
-
 Streptomyces sp.
NADH
-
 Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.6
-
 3-Phenyl-1-propanol pH 8.0, 30°C Streptomyces sp.
15.6
-
 cinnamaldehyde pH 8.0, 30°C Streptomyces sp.
66.5
-
 allyl alcohol pH 8.0, 30°C Streptomyces sp.
106
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
387
-
 NAD+ pH 8.0, 30°C Streptomyces sp.