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Literature summary for 1.1.1.195 extracted from

  • Pennacchio, A.; Rossi, M.; Raia, C.A.
    Synthesis of cinnamyl alcohol from cinnamaldehyde with Bacillus stearothermophilus alcohol dehydrogenase as the isolated enzyme and in recombinant E. coli cells (2013), Appl. Biochem. Biotechnol., 170, 1482-1490.
    View publication on PubMed

Application

Application Comment Organism
synthesis synthesis of cinnamyl alcohol from cinnamaldehyde using the isolated enzyme or by expression of enzyme in Escherichia coli. The reduction of cinnamaldehyde by the isolated enzyme occurrs in 3 h at 50°C with 97% conversion, and yields high purity cinnamyl alcohol with a yield of 88% and a productivity of 50 g/g enzyme. The reduction of 12.5 g/l cinnamaldehyde by whole cells in 6 h, at 37 °C and no requirement of external cofactor occurrs with 97% conversion, 82% yield of 98% pure alcohol and a productivity of 34 mg/g wet cell weight Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
NADH pH 7.5, 50°C Geobacillus stearothermophilus
0.03
-
cinnamaldehyde pH 7.5, 50°C Geobacillus stearothermophilus
0.11
-
cinnamyl alcohol pH 8.8, 50°C Geobacillus stearothermophilus
0.45
-
NAD+ pH 8.8, 50°C Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamaldehyde + NADH + H+ Geobacillus stearothermophilus
-
cinnamyl alcohol + NAD+
-
r
cinnamyl alcohol + NAD+ Geobacillus stearothermophilus
-
cinnamaldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamaldehyde + NADH + H+
-
Geobacillus stearothermophilus cinnamyl alcohol + NAD+
-
r
cinnamyl alcohol + NAD+
-
Geobacillus stearothermophilus cinnamaldehyde + NADH + H+
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
NAD+ pH 8.8, 50°C Geobacillus stearothermophilus
43
-
NADH pH 7.5, 50°C Geobacillus stearothermophilus
43
-
cinnamyl alcohol pH 8.8, 50°C Geobacillus stearothermophilus
55
-
cinnamaldehyde pH 7.5, 50°C Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
73
-
NAD+ pH 8.8, 50°C Geobacillus stearothermophilus
391
-
cinnamyl alcohol pH 8.8, 50°C Geobacillus stearothermophilus
1835
-
cinnamaldehyde pH 7.5, 50°C Geobacillus stearothermophilus
2687
-
NADH pH 7.5, 50°C Geobacillus stearothermophilus