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Literature summary for 1.1.1.195 extracted from

  • Mee, B.; Kelleher, D.; Frias, J.; Malone, R.; Tipton, K.F.; Henehan, G.T.; Windle, H.J.
    Characterization of cinnamyl alcohol dehydrogenase of Helicobacter pylori. An aldehyde dismutating enzyme (2005), FEBS J., 272, 1255-1264.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CAD or HP1140 from strain 26995, cloning in Escherichia coli strain DH5alpha, and expression in strain BL21(DE3) as N-terminally His-tagged enzyme Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
benzyl alcohol competitive mechanism, substrate inhibition Helicobacter pylori
cinnamyl alcohol competitive mechanism, substrate inhibition Helicobacter pylori
coniferyl alcohol competitive mechanism, substrate inhibition Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
cinnamyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
0.008
-
coniferyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
0.03
-
benzaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
0.04
-
acetaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
0.06
-
NADP+ pH 7.5, 37°C, recombinant enzyme, with benzyla alcohol Helicobacter pylori
0.1
-
cinnamyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
0.11
-
coniferyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
0.15
-
NADPH pH 7.5, 37°C, recombinant enzyme, with acetaldehyde Helicobacter pylori
0.41
-
benzyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
9
-
butanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
13
-
Propanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
31
-
benzaldehyde pH 7.5, 37°C, recombinant enzyme, dismutase reaction with NADP+ Helicobacter pylori
46
-
ethanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol predominantly Helicobacter pylori 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamyl alcohol + NADP+ Helicobacter pylori
-
cinnamyl aldehyde + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
strains 1061, 26695, and G27, gene CAD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Helicobacter pylori

Storage Stability

Storage Stability Organism
-20°C, purified recombinant His-tagged enzyme, 75 mM sodium phosphate, pH 7.5, 5 mM DTT, no loss of activity after 1 month Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NADP+ the enzyme also shows dismutase activity Helicobacter pylori benzoic acid + NADPH
-
?
benzyl alcohol + NADP+
-
Helicobacter pylori benzaldehyde + NADPH
-
r
butanol + NADP+
-
Helicobacter pylori butyraldehyde + NADPH
-
r
cinnamyl alcohol + NADP+
-
Helicobacter pylori cinnamyl aldehyde + NADPH
-
r
coniferyl alcohol + NADP+
-
Helicobacter pylori coniferyl aldehyde + NADPH + H+
-
r
ethanol + NADP+
-
Helicobacter pylori acetaldehyde + NADPH + H+
-
r
propanol + NADP+
-
Helicobacter pylori propionaldehyde + NADPH
-
r

Synonyms

Synonyms Comment Organism
CAD
-
Helicobacter pylori
cinnamyl alcohol dehydrogenase
-
Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
benzaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
2.5
-
benzaldehyde pH 7.5, 37°C, recombinant enzyme, dismutase reaction with NADP+ Helicobacter pylori
3.5
-
coniferyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
5.7
-
butanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
7.1
-
ethanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
7.7
-
cinnamyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
8.4
-
benzyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
12.8
-
Propanol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
13.3
-
cinnamyl alcohol pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
15.5
-
NADP+ pH 7.5, 37°C, recombinant enzyme, with benzyla alcohol Helicobacter pylori
16.7
-
acetaldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori
25.2
-
NADPH pH 7.5, 37°C, recombinant enzyme, with acetaldehyde Helicobacter pylori
27.4
-
coniferyl aldehyde pH 7.5, 37°C, recombinant enzyme Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Helicobacter pylori
NADPH
-
Helicobacter pylori