Cloned (Comment) | Organism |
---|---|
- |
Eucalyptus gunnii |
Protein Variants | Comment | Organism |
---|---|---|
S212D | site-directed mutagenesis and overexpression in Escherichia coli | Eucalyptus gunnii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 2',5'-diphosphate | - |
Eucalyptus gunnii | |
adenosine 5'-monophosphate | IC50: 1.58 mM with NADP+ and 0.74 mM with NAD+ | Eucalyptus gunnii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
coniferyl alcohol | with NADP+ | Eucalyptus gunnii | |
0.0038 | - |
NADP+ | - |
Eucalyptus gunnii | |
0.0058 | - |
NADPH | - |
Eucalyptus gunnii | |
0.241 | - |
coniferyl alcohol | with NAD+ | Eucalyptus gunnii | |
1.23 | - |
NAD+ | - |
Eucalyptus gunnii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Eucalyptus gunnii | enzyme involved in lignification in vascular plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eucalyptus gunnii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
recombinant enzyme, with NAD+ as cofactor | Eucalyptus gunnii |
0.39 | - |
S212D mutant, with NAD+ as cofactor | Eucalyptus gunnii |
1.67 | - |
S212D mutant, with NADP+ as cofactor | Eucalyptus gunnii |
4.7 | - |
recombinant enzyme, with NADP+ as cofactor | Eucalyptus gunnii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + NADH + H+ | - |
Eucalyptus gunnii | cinnamyl alcohol + NAD+ | - |
? | |
coniferyl alcohol + NADP+ | - |
Eucalyptus gunnii | coniferyl aldehyde + NADPH + H+ | - |
? | |
additional information | enzyme involved in lignification in vascular plants | Eucalyptus gunnii | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0242 | - |
NAD+ | - |
Eucalyptus gunnii | |
0.0555 | - |
NADP+ | - |
Eucalyptus gunnii | |
0.49 | - |
NADPH | - |
Eucalyptus gunnii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Eucalyptus gunnii | |
NADP+ | - |
Eucalyptus gunnii | |
NADPH | - |
Eucalyptus gunnii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
adenosine 2',5'-diphosphate | with NAD+ | Eucalyptus gunnii | |
0.1 | - |
adenosine 2',5'-diphosphate | with NADP+ | Eucalyptus gunnii | |
0.45 | - |
adenosine 2',5'-diphosphate | with NADH | Eucalyptus gunnii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.58 | - |
IC50: 1.58 mM with NADP+ and 0.74 mM with NAD+ | Eucalyptus gunnii | adenosine 5'-monophosphate |