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Literature summary for 1.1.1.195 extracted from

  • Lauvergeat, V.; Kennedy, K.; Feuillet, C.; McKie, J.H.; Gorrichon, L.; Baltas, M.; Boudet, A.M.; Grima-Pettenati, J.; Douglas, K.T.
    Site-directed mutagenesis of a serine residue in cinnamyl alcohol dehydrogenase, a plant NADPH-dependent dehydrogenase, affects the specificity for the coenzyme (1995), Biochemistry, 34, 12426-12434.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Eucalyptus gunnii

Protein Variants

Protein Variants Comment Organism
S212D site-directed mutagenesis and overexpression in Escherichia coli Eucalyptus gunnii

Inhibitors

Inhibitors Comment Organism Structure
adenosine 2',5'-diphosphate
-
Eucalyptus gunnii
adenosine 5'-monophosphate IC50: 1.58 mM with NADP+ and 0.74 mM with NAD+ Eucalyptus gunnii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
coniferyl alcohol with NADP+ Eucalyptus gunnii
0.0038
-
NADP+
-
Eucalyptus gunnii
0.0058
-
NADPH
-
Eucalyptus gunnii
0.241
-
coniferyl alcohol with NAD+ Eucalyptus gunnii
1.23
-
NAD+
-
Eucalyptus gunnii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Eucalyptus gunnii enzyme involved in lignification in vascular plants ?
-
?

Organism

Organism UniProt Comment Textmining
Eucalyptus gunnii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
recombinant enzyme, with NAD+ as cofactor Eucalyptus gunnii
0.39
-
S212D mutant, with NAD+ as cofactor Eucalyptus gunnii
1.67
-
S212D mutant, with NADP+ as cofactor Eucalyptus gunnii
4.7
-
recombinant enzyme, with NADP+ as cofactor Eucalyptus gunnii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamaldehyde + NADH + H+
-
Eucalyptus gunnii cinnamyl alcohol + NAD+
-
?
coniferyl alcohol + NADP+
-
Eucalyptus gunnii coniferyl aldehyde + NADPH + H+
-
?
additional information enzyme involved in lignification in vascular plants Eucalyptus gunnii ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0242
-
NAD+
-
Eucalyptus gunnii
0.0555
-
NADP+
-
Eucalyptus gunnii
0.49
-
NADPH
-
Eucalyptus gunnii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Eucalyptus gunnii
NADP+
-
Eucalyptus gunnii
NADPH
-
Eucalyptus gunnii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
adenosine 2',5'-diphosphate with NAD+ Eucalyptus gunnii
0.1
-
adenosine 2',5'-diphosphate with NADP+ Eucalyptus gunnii
0.45
-
adenosine 2',5'-diphosphate with NADH Eucalyptus gunnii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.58
-
IC50: 1.58 mM with NADP+ and 0.74 mM with NAD+ Eucalyptus gunnii adenosine 5'-monophosphate