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Literature summary for 1.1.1.194 extracted from

  • Nishimura, M.
    Molecular cloning and expression of the Streptomyces coniferyl alcohol dehydrogenase gene in Escherichia coli (2013), Protein Expr. Purif., 89, 109-115.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
coniferyl alcohol recombinant enzyme, at pH 8.5 and 40°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
4 * 39000, SDS-PAGE Streptomyces sp.
150000
-
gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. M5AJW4
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Resource PHE column chromatography, and Mono Q Superdex 200 column chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
cell-free extract, at pH 8.5 and 40°C Streptomyces sp.
10.7
-
after 40fold purification, at pH 8.5 and 40°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl alcohol + NADP+
-
Streptomyces sp. coniferyl aldehyde + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 39000, SDS-PAGE Streptomyces sp.
homotetramer 4 * 37845, calculated from amino acid sequence Streptomyces sp.

Synonyms

Synonyms Comment Organism
CADH
-
Streptomyces sp.
coniferyl alcohol dehydrogenase
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.8
-
coniferyl alcohol recombinant enzyme, at pH 8.5 and 40°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
93.3
-
coniferyl alcohol recombinant enzyme, at pH 8.5 and 40°C Streptomyces sp.