Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hydroxymethylindole | 42% activation at 0.2 mM | Phycomyces blakesleeanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 59% inhibition at 1 mM | Phycomyces blakesleeanus | |
Hg2+ | 50% inhibition at 0.01 mM | Phycomyces blakesleeanus | |
Indole-3-aldehyde | slight inhibition at 0.2 mM | Phycomyces blakesleeanus | |
indole-3-glycolate | 62% inhibition at 0.2 mM | Phycomyces blakesleeanus | |
KI | 23% inhibition at 1 mM | Phycomyces blakesleeanus | |
Mn2+ | 46% inhibition at 1 mM | Phycomyces blakesleeanus | |
NADP+ | non-competitive inhibition, half-maximal inhibition at 0.2 mM | Phycomyces blakesleeanus | |
Zn2+ | 36% inhibition at 1 mM | Phycomyces blakesleeanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADPH | - |
Phycomyces blakesleeanus | |
0.2 | - |
Indole-3-acetaldehyde | - |
Phycomyces blakesleeanus | |
0.645 | - |
dihydroxyacetone phosphate | - |
Phycomyces blakesleeanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 22% activation at 0.1 mM | Phycomyces blakesleeanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-acetaldehyde + NADPH | Phycomyces blakesleeanus | - |
indole-3-ethanol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phycomyces blakesleeanus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Phycomyces blakesleeanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | maximum enzyme activity from cultures after 48 h | Phycomyces blakesleeanus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
light-grown cells | Phycomyces blakesleeanus |
0.11 | - |
dark-grown cells | Phycomyces blakesleeanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADPH + H+ | very low activity | Phycomyces blakesleeanus | ethanol + NADP+ | - |
? | |
benzaldehyde + NADPH | 2.5fold higher activity than for indole-3-acetaldehyde | Phycomyces blakesleeanus | benzyl alcohol + NADP+ | - |
? | |
dihydroxyacetonephosphate + NADPH | well accepted substrate | Phycomyces blakesleeanus | glycerol 1-phosphate + NADP+ | - |
? | |
indole-3-acetaldehyde + NADPH | - |
Phycomyces blakesleeanus | indole-3-ethanol + NADP+ | - |
? | |
indole-3-aldehyde + NADPH | 19% of the activity of indole-3-acetaldehyde | Phycomyces blakesleeanus | indole-3-ol + NADP+ | - |
? | |
phenylacetaldehyde + NADPH + H+ | well accepted substrate | Phycomyces blakesleeanus | phenylethanol + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Phycomyces blakesleeanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Phycomyces blakesleeanus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | with NAD+ 10times lower activity | Phycomyces blakesleeanus |