Cloned (Comment) | Organism |
---|---|
gene PGFS, quantitative expression analysis, recombinant expression of His-tagged canine-specific PGFS (AKR1C3) in Escherichia coli | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prostaglandin H2 + NADP+ | Canis lupus familiaris | - |
prostaglandin F2alpha + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | Q5FYA7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged canine-specific PGFS (AKR1C3) from Escherichia coli | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | quantitative enzyme expression analysis during pregnancy and prepartum | Canis lupus familiaris | - |
trophoblast | - |
Canis lupus familiaris | - |
uterus | utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation. Colocalization of 15-hydroxyprostaglandin dehydrogenase (HPGD) with PGFS (AKR1C3) expression. Uterine PGFS (AKR1C3) is up-regulated postimplantation and declined prepartum | Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity towards prostaglandin E2 synthesis | Canis lupus familiaris | ? | - |
? | |
prostaglandin H2 + NADP+ | - |
Canis lupus familiaris | prostaglandin F2alpha + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1C3 | - |
Canis lupus familiaris |
PGF2alpha-synthase | - |
Canis lupus familiaris |
PGFS | - |
Canis lupus familiaris |
prostaglandin F synthase | UniProt | Canis lupus familiaris |
prostaglandin F2alpha-synthase | - |
Canis lupus familiaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Canis lupus familiaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Canis lupus familiaris |
General Information | Comment | Organism |
---|---|---|
metabolism | availability of prostaglandins can be regulated by changes in 15-hydroxyprostaglandin dehydrogenase, HPGD, an enzyme that catabolizes prostaglandin E2 and prostaglandin F2alpha to their inactive metabolites 13,14-dihydro-15keto-PGF2alpha and 13,14-dihydro-15-keto prostaglandin E2 (PGEM) | Canis lupus familiaris |
physiological function | the utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation, suggesting a possible role for PGFS (AKR1C3) in the trophoblast invasion | Canis lupus familiaris |