Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | activity of the purified recombinant enzyme is stimulated about 4fold by 1.5 M | Sus scrofa |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Sus scrofa |
wild-type and mutants, plasmid pCold II-PM/PGFS expressed in Escherichia coli BL21 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C44S | retains 63% of the enzyme activity of the wild-type | Mus musculus |
C44S/C47S | shows values below 1% of the enzyme activity of the wild-type | Mus musculus |
C47S | shows values below 1% of the enzyme activity of the wild-type | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0069 | - |
prostaglandin H2 | pH 7.0, 24°C | Mus musculus | |
0.007 | - |
prostaglandin H2 | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
endoplasmic reticulum | - |
Mus musculus | 5783 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
immunoprecipitation, Western blot analysis | Sus scrofa |
23000 | - |
by zinc staining | Mus musculus |
23230 | - |
expressed protein | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Sus scrofa | A9CQL8 | - |
- |
Purification (Comment) | Organism |
---|---|
on Ni2+ column, 102- and 152fold purification of the prostamide F synthase and PGF synthase activities, respectively, achieved from the swine brain (90 g) with a yield of 1.4 and 2%, respectively. Mutants expressed to homogeneity | Sus scrofa |
to apparent homogeneity on Ni2+ column | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Mus musculus | - |
brain | highest activity | Sus scrofa | - |
brain | high activity | Mus musculus | - |
cecum | low activity | Mus musculus | - |
eye | - |
Sus scrofa | - |
eye | low activity | Mus musculus | - |
heart | - |
Sus scrofa | - |
heart | high activity | Mus musculus | - |
kidney | low activity | Mus musculus | - |
large intestine | low activity | Mus musculus | - |
liver | low activity | Mus musculus | - |
lung | - |
Sus scrofa | - |
lung | low activity | Mus musculus | - |
additional information | low activity in deferent duct and epididymidis | Mus musculus | - |
muscle | low activity | Mus musculus | - |
ovary | high activity | Mus musculus | - |
small intestine | low activity | Mus musculus | - |
spinal cord | - |
Mus musculus | - |
spleen | low activity | Mus musculus | - |
stomach | low activity | Mus musculus | - |
testis | low activity | Mus musculus | - |
thymus | high activity | Mus musculus | - |
uterus | high activity | Mus musculus | - |
vesicular gland | low activity | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.062 | - |
PGF synthase activity of the purified enzyme | Sus scrofa |
0.69 | - |
PGF synthase activity of the purified enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with prostaglandin D2 and prostaglandin E2 | Mus musculus | ? | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Mus musculus | prostaglandin F2alpha + NADP+ | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Sus scrofa | prostaglandin F2alpha + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Sus scrofa |
dimer | 2 * 23000, wild-type and mutants, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
prostamide/PGF synthase | - |
Mus musculus |
prostamide/PGF synthase | - |
Sus scrofa |
prostamide/prostaglandin F synthase | - |
Mus musculus |
prostamide/prostaglandin F synthase | - |
Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2467 | - |
prostaglandin H2 | - |
Sus scrofa | |
0.2467 | - |
prostaglandin H2 | pH 7.0, 24°C | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus | |
NADPH | - |
Sus scrofa |
General Information | Comment | Organism |
---|---|---|
physiological function | prostamide/PGF synthase may play an important functional role in the central nervous system | Mus musculus |
physiological function | prostamide/PGF synthase may play an important functional role in the central nervous system. It directly synthesizes prostamide/PGF2alpha from prostamide/PGH2 better than PGF synthase | Sus scrofa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.85 | - |
prostaglandin H2 | pH 7.0, 24°C | Mus musculus |