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Literature summary for 1.1.1.188 extracted from

  • Watanabe, K.; Fujii, Y.; Ohkubo, H.; Kuramitsu, S.; Kagamiyama, H.; Nakanishi, S.; Hayaishi, O.
    Expression of bovine lung prostaglandin F synthase in Escherichia coli (1991), Biochem. Biophys. Res. Commun., 181, 272-278.
    View publication on PubMed
Search for more literature for 1.1.1.188

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 9,10-phenanthrenequinone expressed lung enzyme Bos taurus
0.008
-
 prostaglandin H2 expressed lung enzyme Bos taurus
0.1
-
 Prostaglandin D2 expressed lung enzyme Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration Bos taurus
36000
-
 SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 two isozymes, lung-type and liver-type
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lung two different active sites Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.115
-
 4-nitrobenzaldehyde reductase activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
additional information two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds Bos taurus ?
-
 ?
prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Bos taurus
monomer 1 * 36000, gel filtration Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Bos taurus