Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | uncompetitive for NADH | Klebsiella aerogenes | |
L-acetoin | probably all L(+)-alpha-hydroxycarbonyls products inhibit uncompetitively at non-saturated concentrations | Klebsiella aerogenes | |
NAD+ | competitive inhibition for NADH | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADH | with ethyl pyruvate | Klebsiella aerogenes | |
0.0062 | - |
NADH | with 2,3-pentadione | Klebsiella aerogenes | |
0.0088 | - |
NADH | with diacetyl | Klebsiella aerogenes | |
0.0097 | - |
NADH | with methylglyoxal | Klebsiella aerogenes | |
1.5 | - |
diacetyl | - |
Klebsiella aerogenes | |
10 | - |
2,3-Pentanedione | - |
Klebsiella aerogenes | |
22 | - |
ethyl pyruvate | - |
Klebsiella aerogenes | |
90 | - |
methylglyoxal | - |
Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+ | ordered bi-bi-mechanism, coenzyme binds as first substrate to the enzyme | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-pentanedione + NADH | - |
Klebsiella aerogenes | L-acetylethylcarbinol + NAD+ | - |
ir | |
diacetyl + NADH | - |
Klebsiella aerogenes | L-acetoin + NAD+ | - |
ir | |
ethyl pyruvate + NAD(P)H | - |
Klebsiella aerogenes | ethyl lactate | - |
? | |
methylglyoxal + NADH | - |
Klebsiella aerogenes | L-lactaldehyde | - |
ir | |
R1-CO-CHOH-R2 + NAD(P)H | 2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol | Klebsiella aerogenes | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r | |
R1-CO-CHOH-R2 + NAD(P)H | much more efficient in dehydrogenase direction | Klebsiella aerogenes | R1-CHOH-CHOH-R2 + NAD(P)+ | - |
r | |
R1-CO-CO-R2 + NAD(P)H | methylglyoxal, diacetyl, 2,3-pentanedione | Klebsiella aerogenes | R1-CO-CHOH-R2 + NAD(P)+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
L-glycol dehydrogenase (NAD+) | - |
Klebsiella aerogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella aerogenes | |
NADH | - |
Klebsiella aerogenes |