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Literature summary for 1.1.1.184 extracted from

  • Carlquist, M.; Frejd, T.; Gorwa-Grauslund, M.F.
    Flavonoids as inhibitors of human carbonyl reductase 1 (2008), Chem. Biol. Interact., 174, 98-108.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sacchaormyces cerevisiae Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
computational docking experiments for four inhibitors. For flavonoid inhibitors, the flavonoid skeleton is the binding part of the molecule and sugar moieties are pointing outward, giving rise to a stabilizing effect Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(2E)-1-(2,4-dihydroxyphenyl)-3-(2',4',6-trihydroxy-5'-[(2E)-3-(4-hydroxyphenyl)prop-2-enoyl]biphenyl-3-yl)prop-2-en-1-one
-
Homo sapiens
3-(2-(2,4-dihydroxyphenyl)-1-[hydroxy(4-hydroxyphenyl)methyl]-2-oxoethyl)-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
Homo sapiens
3-[2,3-dihydroxy-4-methoxy-5-(3-methylbut-2-en-1-yl)phenyl]-5,7-dihydroxy-4H-chromen-4-one
-
Homo sapiens
3-[2-(2,4-dihydroxyphenyl)-1-(4-hydroxybenzyl)-2-oxoethyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
-
Homo sapiens
7-O-beta-D-glucopyranos2-ulosyl-quercetin
-
Homo sapiens
quercetin
-
Homo sapiens
quercitrin
-
Homo sapiens
rutin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0852
-
daunorubicin pH 7.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P16152 isoform CBR1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
161.6
-
substrate daunorubicin, pH 7.4, 37°C Homo sapiens
1533
-
substrate 2,3-butanedione, pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanedione + NADPH
-
Homo sapiens ? + NADP+
-
?
2-hydroxy-3-butanone + NADPH
-
Homo sapiens 2,3-dihydroxybutanol + NADP+
-
?
daunorubicin + NADPH
-
Homo sapiens daunorubicinol + NADP+
-
?
doxorubicin + NADPH
-
Homo sapiens doxorubicinol + NADP+
-
?
ethyl acetoacetate + NADPH
-
Homo sapiens ? + NADP+
-
?
menadione + NADPH
-
Homo sapiens ? + NADP+
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0011
-
pH 7.4, 37°C Homo sapiens 3-[2-(2,4-dihydroxyphenyl)-1-(4-hydroxybenzyl)-2-oxoethyl]-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
0.0021
-
pH 7.4, 37°C Homo sapiens rutin
0.0036
-
pH 7.4, 37°C Homo sapiens 3-(2-(2,4-dihydroxyphenyl)-1-[hydroxy(4-hydroxyphenyl)methyl]-2-oxoethyl)-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one
0.0042
-
pH 7.4, 37°C Homo sapiens (2E)-1-(2,4-dihydroxyphenyl)-3-(2',4',6-trihydroxy-5'-[(2E)-3-(4-hydroxyphenyl)prop-2-enoyl]biphenyl-3-yl)prop-2-en-1-one
0.0061
-
pH 7.4, 37°C Homo sapiens 3-[2,3-dihydroxy-4-methoxy-5-(3-methylbut-2-en-1-yl)phenyl]-5,7-dihydroxy-4H-chromen-4-one
0.0062
-
pH 7.4, 37°C Homo sapiens quercetin
0.0065
-
pH 7.4, 37°C Homo sapiens quercitrin
0.008
-
pH 7.4, 37°C Homo sapiens 7-O-beta-D-glucopyranos2-ulosyl-quercetin