Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Sporidiobolus salmonicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporidiobolus salmonicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) partially by ammonium sulfate fractionation | Sporidiobolus salmonicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ | substrate specificity, enantioselectivity, and enzyme-substrate docking studies, Ser133 and Tyr177 are important | Sporidiobolus salmonicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
recombinant enzyme, substrate phenyl 3-oxobutyrate | Sporidiobolus salmonicolor |
0.12 | - |
recombinant enzyme, substrate trifluoromethyl 3-oxobutyrate | Sporidiobolus salmonicolor |
0.18 | - |
recombinant enzyme, substrate tert-butyl 3-oxobutyrate | Sporidiobolus salmonicolor |
0.2 | - |
recombinant enzyme, substrate ethyl 3-oxobutyrate | Sporidiobolus salmonicolor |
0.8 | - |
recombinant enzyme, substrate isopropyl 3-oxobutyrate | Sporidiobolus salmonicolor |
1.4 | - |
recombinant enzyme, substrate chloromethyl 3-oxobutyrate | Sporidiobolus salmonicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-adamantyl methyl ketone + NADPH + H+ | reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity | Sporidiobolus salmonicolor | 1-adamantyl methyl alcohol + NADP+ | - |
? | |
2'-methoxyacetophenone + NADPH + H+ | reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity | Sporidiobolus salmonicolor | 1-(2-methoxyphenyl)ethanol + NADP+ | - |
? | |
chloromethyl 3-oxobutyrate + NADPH + H+ | - |
Sporidiobolus salmonicolor | chloromethyl (S)-3-hydroxybutyrate + NADP+ | 97% purity of the S-enantiomer | ? | |
ethyl 3,3-dimethyl-2-oxobutanoate + NADPH + H+ | reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity | Sporidiobolus salmonicolor | ethyl 3,3-dimethyl-2-hydroxybutanoate + NADP+ | - |
? | |
ethyl 3-oxobutyrate + NADPH + H+ | - |
Sporidiobolus salmonicolor | ethyl (R,S)-3-hydroxybutyrate + NADP+ | 61% S-enantiomer | ? | |
ethyl 3-oxopentanoate + NADPH + H+ | - |
Sporidiobolus salmonicolor | ethyl (R,S)-3-hydroxypentanoate + NADP+ | 61% S-enantiomer | ? | |
ethyl 4,4,4-trifluoro-3-oxobutanoate + NADPH + H+ | - |
Sporidiobolus salmonicolor | ethyl (3S)-4,4,4-trifluoro-3-hydroxybutyrate + NADP+ | 90% purity of the S-enantiomer | ? | |
ethyl 4,4-dimethyl-3-oxopentanoate + NADPH + H+ | reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity | Sporidiobolus salmonicolor | ethyl 4,4-dimethyl-3-hydroxypentanoate + NADP+ | - |
? | |
isopropyl 3-oxobutyrate + NADPH + H+ | - |
Sporidiobolus salmonicolor | isopropyl (S)-3-hydroxybutyrate + NADP+ | 99% purity of the S-enantiomer | ? | |
additional information | broad substrate specificity, overview | Sporidiobolus salmonicolor | ? | - |
? | |
phenyl 3-oxobutyrate + NADPH + H+ | - |
Sporidiobolus salmonicolor | phenyl (S,R)-3-hydroxybutyrate + NADP+ | 56% S-enantiomer | ? | |
tert-butyl 3-oxobutyrate + NADPH + H+ | - |
Sporidiobolus salmonicolor | tert-butyl (S)-3-hydroxybutyrate + NADP+ | 99% purity of the S-enantiomer | ? |
Synonyms | Comment | Organism |
---|---|---|
SSCR | - |
Sporidiobolus salmonicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Sporidiobolus salmonicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Sporidiobolus salmonicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sporidiobolus salmonicolor |