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Literature summary for 1.1.1.184 extracted from

  • Zhu, D.; Yang, Y.; Buynak, J.D.; Hua, L.
    Stereoselective ketone reduction by a carbonyl reductase from Sporobolomyces salmonicolor. Substrate specificity, enantioselectivity and enzyme-substrate docking studies (2006), Org. Biomol. Chem., 4, 2690-2695.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Sporidiobolus salmonicolor

Organism

Organism UniProt Comment Textmining
Sporidiobolus salmonicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) partially by ammonium sulfate fractionation Sporidiobolus salmonicolor

Reaction

Reaction Comment Organism Reaction ID
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ substrate specificity, enantioselectivity, and enzyme-substrate docking studies, Ser133 and Tyr177 are important Sporidiobolus salmonicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
recombinant enzyme, substrate phenyl 3-oxobutyrate Sporidiobolus salmonicolor
0.12
-
recombinant enzyme, substrate trifluoromethyl 3-oxobutyrate Sporidiobolus salmonicolor
0.18
-
recombinant enzyme, substrate tert-butyl 3-oxobutyrate Sporidiobolus salmonicolor
0.2
-
recombinant enzyme, substrate ethyl 3-oxobutyrate Sporidiobolus salmonicolor
0.8
-
recombinant enzyme, substrate isopropyl 3-oxobutyrate Sporidiobolus salmonicolor
1.4
-
recombinant enzyme, substrate chloromethyl 3-oxobutyrate Sporidiobolus salmonicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-adamantyl methyl ketone + NADPH + H+ reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity Sporidiobolus salmonicolor 1-adamantyl methyl alcohol + NADP+
-
?
2'-methoxyacetophenone + NADPH + H+ reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity Sporidiobolus salmonicolor 1-(2-methoxyphenyl)ethanol + NADP+
-
?
chloromethyl 3-oxobutyrate + NADPH + H+
-
Sporidiobolus salmonicolor chloromethyl (S)-3-hydroxybutyrate + NADP+ 97% purity of the S-enantiomer ?
ethyl 3,3-dimethyl-2-oxobutanoate + NADPH + H+ reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity Sporidiobolus salmonicolor ethyl 3,3-dimethyl-2-hydroxybutanoate + NADP+
-
?
ethyl 3-oxobutyrate + NADPH + H+
-
Sporidiobolus salmonicolor ethyl (R,S)-3-hydroxybutyrate + NADP+ 61% S-enantiomer ?
ethyl 3-oxopentanoate + NADPH + H+
-
Sporidiobolus salmonicolor ethyl (R,S)-3-hydroxypentanoate + NADP+ 61% S-enantiomer ?
ethyl 4,4,4-trifluoro-3-oxobutanoate + NADPH + H+
-
Sporidiobolus salmonicolor ethyl (3S)-4,4,4-trifluoro-3-hydroxybutyrate + NADP+ 90% purity of the S-enantiomer ?
ethyl 4,4-dimethyl-3-oxopentanoate + NADPH + H+ reduction of the sterically bulky ketone to the corresponding alcohol with excellent optical purity Sporidiobolus salmonicolor ethyl 4,4-dimethyl-3-hydroxypentanoate + NADP+
-
?
isopropyl 3-oxobutyrate + NADPH + H+
-
Sporidiobolus salmonicolor isopropyl (S)-3-hydroxybutyrate + NADP+ 99% purity of the S-enantiomer ?
additional information broad substrate specificity, overview Sporidiobolus salmonicolor ?
-
?
phenyl 3-oxobutyrate + NADPH + H+
-
Sporidiobolus salmonicolor phenyl (S,R)-3-hydroxybutyrate + NADP+ 56% S-enantiomer ?
tert-butyl 3-oxobutyrate + NADPH + H+
-
Sporidiobolus salmonicolor tert-butyl (S)-3-hydroxybutyrate + NADP+ 99% purity of the S-enantiomer ?

Synonyms

Synonyms Comment Organism
SSCR
-
Sporidiobolus salmonicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Sporidiobolus salmonicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Sporidiobolus salmonicolor

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Sporidiobolus salmonicolor