Application | Comment | Organism |
---|---|---|
synthesis | enzyme is useful for production of ethyl (S)-4-chloro-3-hydroxybutanoate, which is used in the synthesis of pharmacologically and biologically important compouds | Kluyveromyces lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Barbital | 18% inhibition at 1 mM | Kluyveromyces lactis | |
Cu2+ | complete inhibition at 1 mM | Kluyveromyces lactis | |
Hg2+ | complete inhibition at 0.01 mM | Kluyveromyces lactis | |
iodoacetamide | 46% inhibition at 1 mM | Kluyveromyces lactis | |
Methyl vinyl ketone | 74% inhibition at 10 mM | Kluyveromyces lactis | |
additional information | no inhibition by EDTA and crotonic acid | Kluyveromyces lactis | |
N-ethylmaleimide | 80% inhibition at 1 mM | Kluyveromyces lactis | |
o-phenanthroline | about 11% inhibition at 1 mM | Kluyveromyces lactis | |
p-chloromercuribenzoate | complete inhibition at 0.05 mM | Kluyveromyces lactis | |
PMSF | about 10% inhibition at 1 mM | Kluyveromyces lactis | |
quercetin | 31.5% inhibition at 0.1 mM | Kluyveromyces lactis | |
Zn2+ | 88% inhibition at 1 mM | Kluyveromyces lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme forms aggregates of high molecular weight above the size exclusion limit of native PAGE of 1300 kDa | Kluyveromyces lactis |
190000 | - |
x * 190000, SDS-PAGE | Kluyveromyces lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl 4-chloroacetoacetate + NADPH + H+ | Kluyveromyces lactis | enantioselective reduction | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
enzyme KLCR1 from strain NRIC 1329 | - |
Purification (Comment) | Organism |
---|---|
380fold to homogeneity by protamine precipitation, solubilization, ammonium sulfate fractionation, solubilization with 0.5 M NaCl, ultrafiltration, butyl-resin chromatography, and gel filtration | Kluyveromyces lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.8 | - |
purified enzyme | Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butanedione + NADPH | low activity | Kluyveromyces lactis | ? + NADP+ | - |
ir | |
2,3-pentanedione + NADPH + H+ | - |
Kluyveromyces lactis | ? + NADP+ | - |
ir | |
ethyl 4-chloroacetoacetate + NADPH + H+ | enantioselective reduction | Kluyveromyces lactis | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
ir | |
ethyl 4-chloroacetoacetate + NADPH + H+ | highly enantioselective reduction | Kluyveromyces lactis | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
ir | |
methyl 4-chloroacetoacetate + NADPH | highly enantioselective reduction | Kluyveromyces lactis | methyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
ir | |
methylglyoxal + NADPH | very low activity | Kluyveromyces lactis | ? + NADP+ | - |
ir | |
additional information | enzyme is a reductase no dehydrogenase, no activity with phenylglyoxal, 2,4-pentanedione, ethyl 3-oxobutanoate, or methyl 3-oxobutanoate, no activity with nitrobenzaldehydes, xylose, isatin, acetone, acetophenone, propionaldehyde, and pyridine 3-aldehyde, enzyme might be an alpha-subunit of the fatty acid synthase due to amino acid sequence identity, but does not show fatty acid synthase activity | Kluyveromyces lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 190000, SDS-PAGE | Kluyveromyces lactis |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Kluyveromyces lactis |
KLCR1 | - |
Kluyveromyces lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Kluyveromyces lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
30 min, over 80% remaining activity | Kluyveromyces lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Kluyveromyces lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | more than 80% activity at pH 6.0 and pH 8.0 | Kluyveromyces lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Kluyveromyces lactis | |
NADPH | dependent on, no reverse reaction with NADP+ as cofactor | Kluyveromyces lactis |