Literature summary for 1.1.1.184 extracted from

Doorn, J.A.; Maser, E.; Blum, A.; Claffey, D.J.; Petersen, D.R.
Human carbonyl reductase catalyzes reduction of 4-oxonon-2-enal (2004), Biochemistry, 43, 13106-13114.

Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment)	Organism
from a liver cDNA library, overexpression in Escherichia coli as His-tagged enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
4-oxonon-2-enal	the substrate is also a mechanism-based inhibitor of the enzyme resulting in 50% inactivation of the enzyme-NADPH complex, presence of cofactor is required for inhibition	Homo sapiens
additional information	no inhibition by NADP+	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.025	-	menadione	pH 7.4, 37°C, recombinant enzyme	Homo sapiens
0.329	-	glutathione-4-oxonon-2-enal	pH 7.4, 37°C, recombinant enzyme	Homo sapiens
0.345	-	4-oxonon-2-enal	pH 7.4, 37°C, recombinant enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-oxonon-2-enal + NADPH + H+	molecular modeling of substrate binding in the active site	Homo sapiens	4-oxononanal + 4-hydroxynon-2-enal + 1-hydroxynon-2-en-4-one + NADP+	product identification, 4-hydroxynon-2-enal is the major product	?
glutathione-4-oxononanal + NADPH + H+	conjugate, molecular modeling of substrate binding in the active site	Homo sapiens	glutathione-4-hydroxynonanal + NADP+	-	?
menadione + NADPH	best substrate	Homo sapiens	?	-	?
additional information	no activity with 1-hydroxynon-2-en-4-one and 4-hydroxynon-2-enal	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
carbonyl reductase	-	Homo sapiens
CR	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.6	-	glutathione-4-oxonon-2-enal	pH 7.4, 37°C, recombinant enzyme	Homo sapiens
220	-	4-oxonon-2-enal	pH 7.4, 37°C, recombinant enzyme	Homo sapiens
246	-	menadione	pH 7.4, 37°C, recombinant enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)