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Literature summary for 1.1.1.18 extracted from
- Purification and properties of Bacillus subtilis inositol dehydrogenase (1979), J. Biol. Chem., 254, 7684-7690.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
NAD+ | - |
Bacillus subtilis |  |
| 18 | - |
myo-inositol | - |
Bacillus subtilis | |
| 56 | - |
alpha-D-glucose | - |
Bacillus subtilis | |
| 167 | - |
D-glucose | - |
Bacillus subtilis | |
| 190 | - |
D-xylose | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
4 * 39000, SDS-PAGE | Bacillus subtilis |
| 160000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + NAD+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34.3 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucopyranose + NAD+ | 4fold lower activity compared to myo-inositol as substrate, does not act with the beta-anomer | Bacillus subtilis | D-gluconate + NADH | - |
? | |
| D-glucose + NAD+ | - |
Bacillus subtilis | D-gluconate + NADH | - |
? | |
| D-xylose + NAD+ | very low activity | Bacillus subtilis | ? + NADH | - |
? | |
| myo-inositol + NAD+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 39000, SDS-PAGE | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
optimal pH-value for stability | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus subtilis | |
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