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Literature summary for 1.1.1.176 extracted from

  • MacDonald, I.A.; Mahony, D.E.; Jellet, J.F.; Meier, C.E.
    NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559 (1977), Biochim. Biophys. Acta, 489, 466-476.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NAD+
-
Eggerthella lenta
0.1
-
7alpha,12alpha-dihydroxy-5beta-cholanoate
-
Eggerthella lenta

Organism

Organism UniProt Comment Textmining
Eggerthella lenta
-
-
-

Storage Stability

Storage Stability Organism
-20°C, lyophilized, 4 months Eggerthella lenta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha,12alpha-dihydroxy-5beta-cholanoate + NAD+
-
Eggerthella lenta 7alpha-hydroxy-12-oxo-5beta-cholanoate + NADH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, inactivation Eggerthella lenta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10.5 oxidation of bile acids Eggerthella lenta

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Eggerthella lenta
NADP+ low activity Eggerthella lenta