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Literature summary for 1.1.1.176 extracted from
- Bile salt 3alpha- and 12alpha-hydroxysteroid dehydrogenases from Eubacterium lentum and related organisms (1979), Appl. Environ. Microbiol., 37, 992-1000.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
3alpha,12alpha-dihydroxy-5beta-cholanoate | - |
Eggerthella lenta |  |
| 0.03 | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester | 3alpha,12alpha-dihydroxy-5beta-cholanoate | Eggerthella lenta | |
| 0.059 | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoate | - |
Eggerthella lenta | |
| 0.17 | - |
3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine | - |
Eggerthella lenta | |
| 0.25 | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine | - |
Eggerthella lenta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
gel filtration | Eggerthella lenta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eggerthella lenta | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 3 months, 5% loss of activity | Eggerthella lenta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester + NADP+ | - |
Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl methyl ester + NADPH + H+ | - |
r | |
| allocholate + NAD+ | - |
Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH | - |
? | |
| allodeoxycholate + NAD+ | - |
Eggerthella lenta | 3alpha-hydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH | - |
? | |
| cholic acid + NAD(P)+ | - |
Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
| deoxycholic acid + NAD(P)+ | i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid | Eggerthella lenta | 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ | - |
? | |
| glycocholic acid + NAD+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine | Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
| glycodeoxycholic acid + NAD+ | i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine | Eggerthella lenta | 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
| taurocholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine | Eggerthella lenta | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | oxidation of cholic acid | Eggerthella lenta |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.5 | - |
Eggerthella lenta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Eggerthella lenta | |
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