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Literature summary for 1.1.1.175 extracted from
- D-Xylose dehydrogenase (1982), Methods Enzymol., 89, 226-228.
General Stability
| General Stability | Organism |
|---|---|
| thermolabile, stabilizing: substrates or high ionic strength | Pseudomonas sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
NAD+ | - |
Pseudomonas sp. |  |
| 0.5 | - |
D-xylose | - |
Pseudomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NAD+ | Pseudomonas sp. | highly specific for both substrates | D-xylonolactone + NADH + H+ | - |
? | |
| D-xylose + NAD+ | Pseudomonas sp. MSU-1 | highly specific for both substrates | D-xylonolactone + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. MSU-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| NAD+-affinity chromatography | Pseudomonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:D-xylose-grown cell | inducible enzyme synthesis by growth in D-xylose-containing medium | Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60.3 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, addition of DTT, several weeks | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NAD+ | highly specific for both substrates | Pseudomonas sp. | D-xylonolactone + NADH + H+ | - |
? | |
| D-xylose + NAD+ | highly specific for both substrates | Pseudomonas sp. MSU-1 | D-xylonolactone + NADH + H+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
half-life: 2 min | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | cannot be replaced by NADP+ | Pseudomonas sp. | |
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Release 2023.1 (January 2023)
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