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Literature summary for 1.1.1.17 extracted from
- Molecular cloning and characterization of mannitol-1-phosphate dehydrogenase from Vibrio cholerae (2011), J. Microbiol. Biotechnol., 21, 914-920.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mtld1, encoded in the mannitol operon, sequence comparison, real-time PCR expression analysis, expression as His6-tagged protein in Escherichia coli strain SG13009 | Vibrio cholerae serotype O1 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 5 and 10 mM concentrations of MgSO4, MnSO4, FeCl3, ZnCl2, CoCl2, and LiCl have no effect on the enzyme activity | Vibrio cholerae serotype O1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | Vibrio cholerae serotype O1 | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
gene mtlD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography | Vibrio cholerae serotype O1 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | gene mtlD is constitutively expressed even when no mannitol wis present in the growth medium | Vibrio cholerae serotype O1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.32 | - |
recombinant enzyme, crude extract, pH 7.5, 30°C, fructose 6-phosphate reduction | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | - |
Vibrio cholerae serotype O1 | D-fructose 6-phosphate + NADH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mannitol-1-phosphate dehydrogenase | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Vibrio cholerae serotype O1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, reduction reaction | Vibrio cholerae serotype O1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the NAD+-binding consensus sequence GxGxxGxGxxG is localized within the highly conserved N-terminal portion of MtlD corresponding to the amino acid residues 9-19 | Vibrio cholerae serotype O1 | |
| NADH | - |
Vibrio cholerae serotype O1 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the medium chain dehydrogenases | Vibrio cholerae serotype O1 |
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