Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | 48% inhibition of fructose 6-phosphate reduction | Streptococcus mutans | |
ADP | 20% inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
ATP | 53% inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
cytidine | 17% inhibition of fructose 6-phosphate reduction | Streptococcus mutans | |
D-fructose 1,6-diphosphate | 12% inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
D-fructose 6-phosphate | 56% inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
GTP | 11% inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
guanosine | 10% inhibition of fructose 6-phosphate reduction | Streptococcus mutans | |
phosphoenolpyruvate | 14% inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
thymidine | 8% inhibition of fructose 6-phosphate reduction | Streptococcus mutans | |
uridine | 16% inhibition of fructose 6-phosphate reduction | Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADH | cosubstrate: D-fructose 6-phosphate | Streptococcus mutans | |
0.06 | - |
NAD+ | cosubstrate: D-mannitol 1-phosphate | Streptococcus mutans | |
0.15 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Streptococcus mutans | |
1.66 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Streptococcus mutans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Streptococcus mutans | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Streptococcus mutans | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps, affinity chromatography | Streptococcus mutans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Streptococcus mutans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
- |
Streptococcus mutans |
Storage Stability | Organism |
---|---|
-80°C, 0.05 M Tris-HCl, pH 7.5, presence of 2-mercaptoethanol, long term storage without loss of activity | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Streptococcus mutans | D-fructose 6-phosphate + NADH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of D-fructose 6-phosphate | Streptococcus mutans |
8.5 | 9 | oxidation of D-mannitol 1-phosphate | Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific | Streptococcus mutans | |
NADH | - |
Streptococcus mutans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
ADP | competitive inhibition with respect to NADH, noncompetitive inhibition with respect to fructose 6-phosphate | Streptococcus mutans | |
2.2 | - |
ATP | strong inhibition of mannitol 1-phosphate oxidation | Streptococcus mutans | |
3.33 | - |
D-fructose 6-phosphate | able to inhibit the forward reaction to 100%, competitive inhibition with respect to mannitol 1-phosphate, noncompetitive inhibition with respect to NAD+ | Streptococcus mutans |