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Literature summary for 1.1.1.17 extracted from
- Mannitol-1-phosphate dehydrogenase from Cryptococcus neoformans is a zinc-containing long-chain alcohol/polyol dehydrogenase (2000), Microbiology, 146, 2705-2713.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Cryptococcus neoformans |  |
| 0.099 | - |
NADH | cosubstrate: D-fructose 6-phosphate | Cryptococcus neoformans | |
| 0.11 | - |
NAD+ | cosubstrate: D-mannitol 1-phosphate | Cryptococcus neoformans | |
| 0.3 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Cryptococcus neoformans | |
| 0.94 | - |
ethanol | cosubstrate: NAD+ | Cryptococcus neoformans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Cryptococcus neoformans | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 2fold activation at 5 mM, no activation of ethanol oxidation | Cryptococcus neoformans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Cryptococcus neoformans |
| 148000 | - |
gel filtration | Cryptococcus neoformans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | Cryptococcus neoformans | - |
D-fructose 6-phosphate + NADH + H+ | - |
r | |
| D-sorbitol 6-phosphate + NAD+ | Cryptococcus neoformans | 38.8% of the activity compared to D-mannitol 1-phosphate | D-glucose 6-phosphate + NADH + H+ | - |
r | |
| ethanol + NAD+ | Cryptococcus neoformans | 56% of the activity compared to D-mannitol 1-phosphate | acetaldehyde + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptococcus neoformans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Cryptococcus neoformans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Cryptococcus neoformans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
crude extract, D-mannitol 1-phosphate oxidation | Cryptococcus neoformans |
| 116 | - |
purified enzyme D-mannitol 1-phosphate oxidation | Cryptococcus neoformans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol 1-phosphate + NAD+ | - |
Cryptococcus neoformans | D-fructose 6-phosphate + NADH + H+ | - |
r | |
| D-sorbitol 6-phosphate + NAD+ | 38.8% of the activity compared to D-mannitol 1-phosphate | Cryptococcus neoformans | D-glucose 6-phosphate + NADH + H+ | - |
r | |
| ethanol + NAD+ | 56% of the activity compared to D-mannitol 1-phosphate | Cryptococcus neoformans | acetaldehyde + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Cryptococcus neoformans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable for one week | Cryptococcus neoformans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | NADH-dependent reduction of D-fructose 6-phosphate | Cryptococcus neoformans |
| 9 | 9.5 | NAD+-dependent oxidation of D-mannitol 1-phosphate | Cryptococcus neoformans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | strictly specific | Cryptococcus neoformans | |
| NADH | - |
Cryptococcus neoformans | |
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