Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
NRRL Y-2034 | - |
Saccharomyces cerevisiae NRRL Y-2034 | - |
NRRL Y-2034 | - |
Purification (Comment) | Organism |
---|---|
gel chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde | Saccharomyces cerevisiae |
0.38 | - |
at pH 6.5 using NADPH and ketopantoic acid | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ketopantoic acid + NADPH | - |
Saccharomyces cerevisiae | pantoic acid + NADP+ | - |
? | |
ketopantoic acid + NADPH | - |
Saccharomyces cerevisiae NRRL Y-2034 | pantoic acid + NADP+ | - |
? | |
additional information | activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde | Saccharomyces cerevisiae | ? | - |
? | |
additional information | activity with ketopantoic acid is 100%, with ketopantoyl lactone 6%, with oxoalacetic acid 4%, no activity with 2-ketoisovaleric acid, pyruvic acid, 3-hydroxypyruvic acid, 3-phosphohydroxypyruvic acid, 2-ketobutyric acid, 2-ketoglutaric acid, and acetaldehyde | Saccharomyces cerevisiae NRRL Y-2034 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of ketopantoic acid with NADPH | Saccharomyces cerevisiae |
5.5 | - |
reduction of ketopantoic acid with NADH | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 6.5 | pH 4.3: about 10% of activity maximum, pH 6.5: about 40% of activity maximum | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | less active than NADPH at 0.1 mM | Saccharomyces cerevisiae | |
NADPH | - |
Saccharomyces cerevisiae |