Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacteroides fragilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | complete inhibition at 7.5 mM | Bacteroides fragilis | |
p-hydroxymercuribenzoate | complete inhibition at 0.0075 mM | Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
chenodeoxycholic acid | pH 8.5, 37°C | Bacteroides fragilis | |
0.177 | - |
cholic acid | pH 8.5, 37°C | Bacteroides fragilis | |
0.212 | - |
NAD+ | with cholic acid as substrate, pH 8.5, 37°C | Bacteroides fragilis | |
0.709 | - |
NAD+ | with chenodeoxycholic acid as substrate, pH 8.5, 37°C | Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27400 | - |
SDS-PAGE | Bacteroides fragilis |
110000 | - |
gel filtration | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | Q9S3U5 | strain ATCC 25285 | - |
Escherichia coli | - |
- |
- |
Paeniclostridium sordellii | - |
strain ATCC 9714 | - |
[Clostridium] scindens | - |
formerly Eubacterium sp. strain VP1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
351 | - |
purified enzyme, 37°C | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholic acid + NAD+ | - |
Escherichia coli | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
chenodeoxycholic acid + NAD+ | - |
Paeniclostridium sordellii | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
chenodeoxycholic acid + NAD+ | - |
[Clostridium] scindens | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
chenodeoxycholic acid + NAD+ | - |
Bacteroides fragilis | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
cholic acid + NAD+ | - |
Escherichia coli | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
cholic acid + NAD+ | - |
Paeniclostridium sordellii | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
cholic acid + NAD+ | - |
[Clostridium] scindens | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
cholic acid + NAD+ | - |
Bacteroides fragilis | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27400, subunit mass by SDS-PAGE, native mass by gel filtration | Bacteroides fragilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
complete loss of activity within 30 min | Escherichia coli |
65 | - |
complete loss of activity within 30 min | Paeniclostridium sordellii |
65 | - |
complete loss of activity within 30 min | [Clostridium] scindens |
65 | - |
95% of initial activity after 1 h | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
NADH oxidation | Bacteroides fragilis |
8.5 | - |
NAD reduction | Bacteroides fragilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | NAD reduction | Bacteroides fragilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NAD+ | - |
Paeniclostridium sordellii | |
NAD+ | - |
[Clostridium] scindens | |
NAD+ | - |
Bacteroides fragilis | |
NADH | - |
Bacteroides fragilis |