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Literature summary for 1.1.1.156 extracted from

  • Marshall, J.H.; Kong, Y.C.; Sloan, J.; May, J.W.
    Purification and properties of glycerol:NADP+ 2-oxidoreductase from Schizisaccharomyces pombe (1989), J. Gen. Microbiol., 135, 697-701.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 80% inhibition at 1 mM Schizosaccharomyces pombe
8-hydroxyquinoline complete inhibition at 10 mM Schizosaccharomyces pombe
dithiothreitol 25% inhibition at 10 mM Schizosaccharomyces pombe
L-cysteine 40% inhibition at 10 mM Schizosaccharomyces pombe
p-chloromercuribenzoate strong inhibition Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NADP+
-
Schizosaccharomyces pombe
0.34
-
NADPH
-
Schizosaccharomyces pombe
0.67
-
dihydroxyacetone
-
Schizosaccharomyces pombe
51
-
glycerol
-
Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 30000 + 1 * 25000, SDS-PAGE Schizosaccharomyces pombe
30000
-
1 * 30000 + 1 * 25000, SDS-PAGE Schizosaccharomyces pombe
57000
-
gel filtration Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NADP+ Schizosaccharomyces pombe
-
dihydroxyacetone + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 1step chromatography Schizosaccharomyces pombe

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:dihydroxyacetone-grown cell
-
Schizosaccharomyces pombe
-
culture condition:glycerol-grown cell
-
Schizosaccharomyces pombe
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.7
-
-
Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3-butanetriol + NADP+ 18% of the activity compared to glycerol Schizosaccharomyces pombe 1,3-dihydroxybutan-2-one + NADPH
-
?
1,2-butanediol + NADP+ 19% of the activity compared to glycerol Schizosaccharomyces pombe 1-hydroxybutan-2-one + NADPH
-
?
1,2-propanediol + NADP+ 30% of the activity compared to glycerol, low reverse reaction rate Schizosaccharomyces pombe hydroxyacetone + NADPH
-
r
2,3-butanediol + NADP+ 25% of the activity compared to glycerol, low reverse reaction rate Schizosaccharomyces pombe 3-hydroxybutan-2-one + NADPH
-
r
glycerol + NADP+
-
Schizosaccharomyces pombe dihydroxyacetone + NADPH + H+
-
r
propan-2-ol + NADP+ very low activity Schizosaccharomyces pombe acetone + NADPH
-
?
pyruvaldehyde + NADPH very low activity Schizosaccharomyces pombe lactaldehyde + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 1 * 30000 + 1 * 25000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
GDH4
-
Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
dihydroxyacetone reduction Schizosaccharomyces pombe
9.5
-
glycerol oxidation Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
NADP+ can not be replaced by NAD+ Schizosaccharomyces pombe