Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 80% inhibition at 1 mM | Schizosaccharomyces pombe | |
8-hydroxyquinoline | complete inhibition at 10 mM | Schizosaccharomyces pombe | |
dithiothreitol | 25% inhibition at 10 mM | Schizosaccharomyces pombe | |
L-cysteine | 40% inhibition at 10 mM | Schizosaccharomyces pombe | |
p-chloromercuribenzoate | strong inhibition | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADP+ | - |
Schizosaccharomyces pombe | |
0.34 | - |
NADPH | - |
Schizosaccharomyces pombe | |
0.67 | - |
dihydroxyacetone | - |
Schizosaccharomyces pombe | |
51 | - |
glycerol | - |
Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 30000 + 1 * 25000, SDS-PAGE | Schizosaccharomyces pombe |
30000 | - |
1 * 30000 + 1 * 25000, SDS-PAGE | Schizosaccharomyces pombe |
57000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NADP+ | Schizosaccharomyces pombe | - |
dihydroxyacetone + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 1step chromatography | Schizosaccharomyces pombe |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:dihydroxyacetone-grown cell | - |
Schizosaccharomyces pombe | - |
culture condition:glycerol-grown cell | - |
Schizosaccharomyces pombe | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.7 | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,3-butanetriol + NADP+ | 18% of the activity compared to glycerol | Schizosaccharomyces pombe | 1,3-dihydroxybutan-2-one + NADPH | - |
? | |
1,2-butanediol + NADP+ | 19% of the activity compared to glycerol | Schizosaccharomyces pombe | 1-hydroxybutan-2-one + NADPH | - |
? | |
1,2-propanediol + NADP+ | 30% of the activity compared to glycerol, low reverse reaction rate | Schizosaccharomyces pombe | hydroxyacetone + NADPH | - |
r | |
2,3-butanediol + NADP+ | 25% of the activity compared to glycerol, low reverse reaction rate | Schizosaccharomyces pombe | 3-hydroxybutan-2-one + NADPH | - |
r | |
glycerol + NADP+ | - |
Schizosaccharomyces pombe | dihydroxyacetone + NADPH + H+ | - |
r | |
propan-2-ol + NADP+ | very low activity | Schizosaccharomyces pombe | acetone + NADPH | - |
? | |
pyruvaldehyde + NADPH | very low activity | Schizosaccharomyces pombe | lactaldehyde + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 30000 + 1 * 25000, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
GDH4 | - |
Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
dihydroxyacetone reduction | Schizosaccharomyces pombe |
9.5 | - |
glycerol oxidation | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | can not be replaced by NAD+ | Schizosaccharomyces pombe |