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Literature summary for 1.1.1.153 extracted from

  • Tang, Y.; Pei, Z.; Liu, L.; Wang, D.; Kong, L.; Liu, S.; Jiang, X.; Gao, Y.; Ma, H.
    Expression and enzyme activity detection of a sepiapterin reductase gene from Musca domestica larva (2016), Appl. Biochem. Biotechnol., 181, 604-612.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
subtractive cDNA library screening, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Musca domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0235
-
sepiapterin pH 6.4, 37°C, recombinant enzyme Musca domestica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
-
Musca domestica

Organism

Organism UniProt Comment Textmining
Musca domestica A0A172EJV6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Musca domestica

Source Tissue

Source Tissue Comment Organism Textmining
body wall larval Musca domestica
-
fat body larval Musca domestica
-
larva
-
Musca domestica
-
malpighian tubule larval Musca domestica
-
midgut larval, high enzyme level Musca domestica
-
additional information during the development of Musca domestica, the expression of MDSPR in the larvae is higher than other developmental stages Musca domestica
-
trachea larval Musca domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sepiapterin + NADPH + H+
-
Musca domestica tetrahydrobiopterin + NADP+ no stereochemic specification in the publication ?

Subunits

Subunits Comment Organism
? x * 51000, soluble recombinant His-tagged enzyme, SDS-PAGE Musca domestica

Synonyms

Synonyms Comment Organism
MDSPR
-
Musca domestica
sepiapterin reductase
-
Musca domestica
SPR
-
Musca domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant enzyme Musca domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 activity range, profile overview, recombinant His-tagged enzyme, over 50% of maximal activity at 30-85°C Musca domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
recombinant enzyme Musca domestica

pH Range

pH Minimum pH Maximum Comment Organism
2.5 12 activity range, profile overview, recombinant His-tagged enzyme, over 50% of maximal activity at pH 3.0-11.0 Musca domestica

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Musca domestica

Expression

Organism Comment Expression
Musca domestica infection with Escherichia coli induces the enzyme in Musca domestica larvae, highest after 24 h up

General Information

General Information Comment Organism
metabolism sepiapterin reductase (SPR) catalyzes the final steps of tetrahydrobiopterin (BH4) biosynthesis Musca domestica
physiological function sepiapterin reductase (SPR) catalyzes the final steps of tetrahydrobiopterin (BH4) biosynthesis. BH4 is an essential cofactor for aromatic acid hydroxylases and nitric oxide synthase. BH4 deficiency causes endothelial dysfunction, such as hypertension, atherosclerosis, diabetes, etc. Musca domestica