KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
2-amino-6-(2-hydroxypropanoyl)pteridin-4(1H)-one | pH 6.4, 25°C | Rattus norvegicus | |
0.02 | - |
benzil | pH 6.4, 25°C | Rattus norvegicus | |
0.0218 | - |
phenylpropanedione | - |
Rattus norvegicus | |
0.0218 | - |
phenylpropanedione | pH 6.4, 25°C | Rattus norvegicus | |
1 | - |
diacetyl | - |
Rattus norvegicus | |
1 | - |
diacetyl | pH 6.4, 25°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | - |
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | Rattus norvegicus | i.e. 2-amino-4-hydroxy-6-lactyl-7,8-dihydropteridine, biosynthesis pathway, overview | 7,8-dihydrobiopterin + NADP+ | i.e. 2-amino-4-hydroxy-6-(1',2'-dihydroxypropyl-)7,8-dihydropteridine, which is a substrate for biosynthesis of tetrahydrobiopterin, i.e. 6R-L-erythro-1',2'-dihydroxy-propyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine, an important component for controling the concentration of catecholamines or indoleamines in the brain and other nerve tissues | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
native enzyme from erythrocytes | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ | enzyme also reduces carbonyl compounds of non-pteridine derivatives | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
erythrocyte | - |
Rattus norvegicus | - |
nerve | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenyl-1,2-propanedione + NADPH | - |
Rattus norvegicus | ? | - |
? | |
2,3-butanediol + NADP+ | - |
Rattus norvegicus | ? | - |
? | |
2-amino-6-(2-hydroxypropanoyl)pteridin-4(1H)-one + NADPH + H+ | - |
Rattus norvegicus | ? | - |
r | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADP+ | - |
Rattus norvegicus | ? | - |
? | |
acetoin + NADPH | 0.003 mM NADPH oxidised per 30 min | Rattus norvegicus | ? | - |
? | |
benzil + NADPH | - |
Rattus norvegicus | ? | - |
? | |
benzil + NADPH | NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus | ? | - |
? | |
diacetyl + NADPH | - |
Rattus norvegicus | ? | - |
? | |
diacetyl + NADPH | NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus | acetoin + NADP+ | - |
? | |
additional information | some monoketotestosterones show slight activity | Rattus norvegicus | ? | - |
? | |
additional information | substrate specificity, the enzyme also exhibits non-specific NADPH-dependent dicarbonyl reductase activity, overview | Rattus norvegicus | ? | - |
? | |
phenylpropanedione + NADPH | NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus | phenylpropan-2,3-diol + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | i.e. 2-amino-4-hydroxy-6-lactyl-7,8-dihydropteridine, biosynthesis pathway, overview | Rattus norvegicus | 7,8-dihydrobiopterin + NADP+ | i.e. 2-amino-4-hydroxy-6-(1',2'-dihydroxypropyl-)7,8-dihydropteridine, which is a substrate for biosynthesis of tetrahydrobiopterin, i.e. 6R-L-erythro-1',2'-dihydroxy-propyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine, an important component for controling the concentration of catecholamines or indoleamines in the brain and other nerve tissues | ? | |
sepiapterin + NADPH + H+ | sepiapterin reductase activity | Rattus norvegicus | 7,8-dihydrobiopterin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SPR | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, sepiapterin reductase activity | Rattus norvegicus |
37 | - |
assay at, NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
assay at, sepiapterin reductase activity | Rattus norvegicus |
6.8 | - |
assay at, NADPH-dependent dicarbonyl reductase activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |