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Literature summary for 1.1.1.151 extracted from

  • Monder, C.; White, A.
    The 21-hydroxysteroid dehydrogenases of liver. A nicotinamide adenine dinucleotide phosphate dehydrogenase and two nicotinamide adenine dinucleotide dehydrogenases (1965), J. Biol. Chem., 240, 71-77.
    View publication on PubMed
Search for more literature for 1.1.1.151

General Stability

General Stability Organism
not sensitive against repeated freezing and thawing Ovis aries aries

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 0.004 mM Ovis aries aries
Hg2+ 53% inhibition at 0.002 mM Ovis aries aries
NADH competitive inhibition Ovis aries aries
NADPH competitive inhibition Ovis aries aries
p-mercuribenzoate 55% inhibition at 0.014 mM Ovis aries aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 NADPH
-
 Ovis aries aries
0.118
-
 pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
0.137
-
 11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
-
 Ovis aries aries
0.137
-
 17-hydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
0.177
-
 11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
0.233
-
 11beta-hydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
0.461
-
 17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
 Ovis aries aries

Organism

Organism UniProt Comment Textmining
Ovis aries aries
-
 sheep
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, Sephadex G-100, starch block elelctrophoresis Ovis aries aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries aries
-

Storage Stability

Storage Stability Organism
-10°C, 0.01 M sodium phosphate , pH 6-7.4, 1 year Ovis aries aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADPH i.e. 21-dehydro-delta1-cortisol Ovis aries aries 11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NADP+ 21-hydroxy-delta1-cortisol ?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydrocortisol Ovis aries aries 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol ?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydrocorticosterone Ovis aries aries 11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+ 21-hydroxycorticosterone ?
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH + H+
-
 Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+
-
 r
17-hydroxy-pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydro-11-deoxycortisol Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. 21-hydroxy-11-deoxycortisol ?
17alpha-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH i.e. 21-dehydrocortisone, marked dependence on the nature of steroids Ovis aries aries 17alpha,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+ i.e. cortisone ?
pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydro-11-deoxy-corticosterone Ovis aries aries 21-hydroxy-pregn-4-ene-3,20-dione + NADP+ 21-hydroxy-11-deoxy-corticosterone ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 80% loss of activity in 5 min Ovis aries aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9 6.1
-
 Ovis aries aries

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Ovis aries aries