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Literature summary for 1.1.1.149 extracted from

  • Naumann, J.M.; Zoellner, A.; Dragan, C.A.; Messinger, J.; Adam, J.; Bureik, M.
    Biotechnological production of 20-alpha-dihydrodydrogesterone at pilot scale (2011), Appl. Biochem. Biotechnol., 165, 190-203.
    View publication on PubMed

Application

Application Comment Organism
biotechnology a process is developed that that allows the production of 20alpha- dihydrodydrogesterone at technical scale (several grams of 20a-DHD per week and fermenter). Genetic improvement of the production strain, an increase of substrate solubility by addition of ß-cyclodextrin, and the development of a sophisticated high-cell density fermentation at pilot scale are employed. By usage of the exemplary substrate progesterone, it is hsown that this innovative fission yeast-based whole cell biotransformation process is transferable to the conversion of other AKR1C1 substrates without special adaptation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in fission yeast cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
progesterone + NADH
-
Homo sapiens 20alpha-hydroxyprogesterone + NAD+
-
?

Synonyms

Synonyms Comment Organism
20alpha-HSD
-
Homo sapiens
20alpha-hydroxysteroid dehydrogenase
-
Homo sapiens
AKR1C1
-
Homo sapiens