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Literature summary for 1.1.1.149 extracted from

  • Couture, J.F.; Legrand, P.; Cantin, L.; Luu-The, V.; Labrie, F.; Breton, R.
    Human 20alpha-hydroxysteroid dehydrogenase: crystallographic and site-directed mutagenesis studies lead to the identification of an alternative binding site for C21-steroids (2003), J. Mol. Biol., 331, 593-604.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
ternary complex with NADP+ and 20alpha-hydroxyprogesterone Homo sapiens

Protein Variants

Protein Variants Comment Organism
D309L similar Km-value as wild-type Homo sapiens
E127D increase in Km-value Homo sapiens
H222I dramatic increase in Km-value Homo sapiens
H222S large increase in Km-value Homo sapiens
R304L dramatic increase in Km-value Homo sapiens
T307V similar Km-value as wild-type Homo sapiens
Y305F similar Km-value as wild-type Homo sapiens
Y55F no enzymic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
NADPH wild type, pH 7.5, 37°C Homo sapiens
0.00117
-
20alpha-hydroxyprogesterone wild type, pH 7.5, 37°C Homo sapiens
0.0021
-
NADPH mutant H222S, pH 7.5, 37°C Homo sapiens
0.0041
-
progesterone mutant T307V, pH 7.5, 37°C Homo sapiens
0.0042
-
progesterone wild type, pH 7.5, 37°C Homo sapiens
0.0046
-
progesterone mutant D309L, pH 7.5, 37°C Homo sapiens
0.0056
-
progesterone mutant Y305F, pH 7.5, 37°C Homo sapiens
0.0094
-
progesterone mutant E127D, pH 7.5, 37°C Homo sapiens
0.019
-
NADPH mutant H222S, pH 7.5, 37°C Homo sapiens
0.0256
-
progesterone mutant H222S, pH 7.5, 37°C Homo sapiens
0.0286
-
progesterone mutant H222I, pH 7.5, 37°C Homo sapiens
0.069
-
dihydrotestosterone wild type, pH 7.5, 37°C Homo sapiens
0.098
-
20alpha-hydroxyprogesterone mutant H222I, pH 7.5, 37°C Homo sapiens
0.151
-
dihydrotestosterone mutant R304L, pH 7.5, 37°C Homo sapiens
0.1774
-
20alpha-hydroxyprogesterone mutant H222S, pH 7.5, 37°C Homo sapiens
0.2833
-
progesterone mutant R304L, pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20alpha-hydroxyprogesterone + NADP+
-
Homo sapiens progesterone + NADPH
-
r
dihydrotestosterone + NADPH
-
Homo sapiens ? + NADP+
-
r
progesterone + NADPH
-
Homo sapiens 20alpha-hydroxyprogesterone + NADP+
-
r

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens