Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha and baculovirus-insect cell systems Sf9 | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | 0.0059 | 20alpha-hydroxyprogesterone | 37°C, 10 min | Rattus norvegicus | |
0.0095 | 0.0096 | NADP+ | 37°C, 7.5 min | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NADPH | Rattus norvegicus | central role in luteolysis and parturition | 20alpha-hydroxyprogesterone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme expressed by insect cells, not essential for catalytic activity | Rattus norvegicus |
phosphoprotein | enzyme expressed by insect cells, enzyme expressed by E. coli phosphorylated by various kinases, not essential for catalytic activity | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
affinity binding to glutathione-Sepharose and thrombin digestion of bacterial lysate, ion exchange and affinity chromatography of enzyme produced by insect cells | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | corpus luteum | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2596 | - |
insect cells | Rattus norvegicus |
0.332 | - |
+/-0.047, Escherichia coli | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Rattus norvegicus | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+ | - |
? | |
4-nitrobenzaldehyde + NADPH | - |
Rattus norvegicus | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
benzaldehyde + NADPH | - |
Rattus norvegicus | benzyl alcohol + NADP+ | - |
? | |
D-galactose + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
D-glucose + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
DL-glyceraldehyde + NADPH | - |
Rattus norvegicus | glycerol + NADP+ | - |
? | |
methylglyoxal + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
additional information | benzaldehyde, DL-glyceraldehyde, 4-nitrobenzaldehyde and glucose very efficient substrates, methylglyoxal, 9,10-phenanthrenquinone and D-galactose less active, no activity with corticosterone and 5alpha-dihydrotestosterone | Rattus norvegicus | ? | - |
? | |
progesterone + NADPH | - |
Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
r | |
progesterone + NADPH | central role in luteolysis and parturition | Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |