Crystallization (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
General Stability | Organism |
---|---|
NADP+ protects against thermal inactivation, 20alpha-hydroxyprogesterone does not | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
17beta-estradiol | 20alpha-hydroxyprogesterone analog, competitive or noncompetitive depending on variable substrate | Rattus norvegicus | |
2'-phosphoadenosine diphosphoribose | NADP+ analog, competitive or noncompetitive depending on variable substrate | Rattus norvegicus | |
3-Aminopyridine adenine dinucleotide phosphate | - |
Rattus norvegicus | |
additional information | no inhibition by NADPH with saturating concentration of NADP+ | Rattus norvegicus | |
NADPH | competitive and noncompetitive product inhibition with respect to NADP+ depending on substrate concentrations | Rattus norvegicus | |
progesterone | noncompetitive product inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
NADP+ | spectrophotometric, pH 6.8 | Rattus norvegicus | |
0.00088 | - |
NADP+ | fluorometric, pH 7.8 | Rattus norvegicus | |
0.00102 | - |
20alpha-hydroxy-4-pregnen-3-one | spectrophotometric, pH 6.8 | Rattus norvegicus | |
0.00138 | - |
NADPH | spectrophotometric, pH 6.8 | Rattus norvegicus | |
0.00162 | - |
20alpha-hydroxy-4-pregnen-3-one | fluorometric, pH 7.8 | Rattus norvegicus | |
0.00186 | - |
thionicotinamide adenine dinucleotide phosphate | NADP analog | Rattus norvegicus | |
0.00208 | - |
3-acetylpyridine adenine dinucleotide phosphate | NADP analog | Rattus norvegicus | |
0.00538 | - |
progesterone | spectrophotometric pH 6.8 | Rattus norvegicus | |
0.0588 | - |
nicotinamide hypoxanthine dinucleotide phosphate | NADP analog | Rattus norvegicus | |
0.1429 | - |
nicotinamide 1,N6-ethenoadenine dinucleotide phosphate | NADP analog | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Rattus norvegicus |
38000 | - |
1 * 38000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female, nonpregnant, DUB:(SD) | - |
Purification (Comment) | Organism |
---|---|
gel filtration, affinity chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ | bibi mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.48 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-15°C, 0.005 mg/ml, 0.01 M potassium phosphate buffer, pH 8.0, 50% propylene glycol, 0.2 M KCl, 2 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20alpha-hydroxypregn-4-en-3-one + 3-acetylpyridine adenine dinucleotide phosphate | - |
Rattus norvegicus | ? | - |
r | |
20alpha-hydroxypregn-4-en-3-one + nicotinamide 1,N6-ethenoadenine dinucleotide phosphate | - |
Rattus norvegicus | ? | - |
r | |
20alpha-hydroxypregn-4-en-3-one + nicotinamide hypoxanthine dinucleotide phosphate | - |
Rattus norvegicus | ? | - |
r | |
20alpha-hydroxypregn-4-en-3-one + thionicotinamide adenine dinucleotide phosphate | - |
Rattus norvegicus | ? | - |
r | |
additional information | no activity with 20alpha-hydroxy-4-pregnen-3-one | Rattus norvegicus | ? | - |
? | |
progesterone + NADPH | - |
Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
NADP+ protects against thermal inactivation | Rattus norvegicus |
25 | - |
20 min | Rattus norvegicus |
38 | - |
half-life 7.6 min | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
varying NADP+ or 20alpha-hydroxyprogesterone concentrations | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide phosphate | coenzyme analog | Rattus norvegicus | |
NADP+ | coenzyme | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus | |
nicotinamide 1,N6-ethenoadenine dinucleotide phosphate | coenzyme analog | Rattus norvegicus | |
nicotinamide hypoxanthine dinucleotide phosphate | coenzyme analog | Rattus norvegicus | |
thio-NADP+ | coenzyme analog | Rattus norvegicus |