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Literature summary for 1.1.1.148 extracted from

  • De Prada, P.; Setchell, K.D.R.; Hylemon, P.B.
    Purification and characterization of a novel 17alpha-hydroxysteroid dehydrogenase from an intestinal Eubacterium sp. VPI 12708 (1994), J. Lipid Res., 35, 922-929.
    View publication on PubMed
Search for more literature for 1.1.1.148

General Stability

General Stability Organism
sensitive to molecular oxygen Eubacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Eubacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 NADP+
-
 Eubacterium sp.
0.005
-
 NADPH
-
 Eubacterium sp.
0.031
-
 4-androsten-3,17-dione
-
 Eubacterium sp.
0.07
-
 Epitestosterone
-
 Eubacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Eubacterium sp.
Zn2+
-
 Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, SDS-PAGE Eubacterium sp.
160000
-
 gel filtration Eubacterium sp.

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
 inducible by growth on androstenedione and cholic acid, not deoxycholic acid
-
Eubacterium sp.
-
 VPI 12708
-
Eubacterium sp. VPI 12708
-
 VPI 12708
-

Purification (Commentary)

Purification (Comment) Organism
-
 Eubacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 oxidation of 17alpha-hydroxy-4-androsten-3-one Eubacterium sp.
3.25
-
 reduction of 4-androsten-3,17-dione Eubacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-estradiol + NAD(P)+
-
 Eubacterium sp. estrone + NAD(P)H
-
 r
17alpha-estradiol + NAD(P)+
-
 Eubacterium sp. VPI 12708 estrone + NAD(P)H
-
 r
17alpha-hydroxy-4-androsten-3-one + NADP+ epitestosterone Eubacterium sp. 4-androsten-3,17-dione + NADPH
-
 r
17alpha-hydroxy-4-androsten-3-one + NADP+ epitestosterone Eubacterium sp. VPI 12708 4-androsten-3,17-dione + NADPH
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE Eubacterium sp.

Synonyms

Synonyms Comment Organism
17alpha-hydroxysteroid dehydrogenase
-
 Eubacterium sp.
More N-terminal sequence comparison of several enzymes: 17alpha-hydroxysteroid dehydrogenase belongs to the disulfide oxidoreductase family, subfamily dihydrolipoamide dehydrogenases Eubacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 reduction of 4-androsten-3,17-dione Eubacterium sp.
6.5
-
 oxidation of 17alpha-hydroxy-4-androsten-3-one Eubacterium sp.

Cofactor

Cofactor Comment Organism Structure
NADP+ highly specific for Eubacterium sp.