Protein Variants | Comment | Organism |
---|---|---|
Y177A | site-directed mutagenesis, the mutant shows increased Km for cortisone compared to the wild-type enzyme, the mutation reduces the sensitivity to glycyrrhetinic acid inhibition 6-10fold, but not to carbenoxolone | Homo sapiens |
Y177F | site-directed mutagenesis, the mutant shows kinetics similar to the wild-type enzyme | Homo sapiens |
Y177Q | site-directed mutagenesis, the mutant shows increased Km for cortisone compared to the wild-type enzyme, the mutation reduces the sensitivity to glycyrrhetinic acid inhibition 6-10fold, but not to carbenoxolone | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carbenoxolone | - |
Homo sapiens | |
glycyrrhetinic acid | isozyme 11beta-HSD1 inhibitor, residue Tyr177 is important in binding, molecular modeling | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | Homo sapiens | isozyme 11beta-HSD1 | cortisol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme 11beta-HSD1 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an 11beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH + H+ | substrate binding and active site structure, isozyme 11beta-HSD1 contains the conserved catalytic motif YSASK, Tyr177 is not a hydrogen bond donor in substrate binding but is involved via hydrophobic interaction, Tyr177 is not involved in determination of substrate specificity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-dehydrocorticosterone + NADPH | isozyme 11beta-HSD1 | Homo sapiens | corticosterone + NADP+ | - |
? | |
cortisone + NADPH + H+ | isozyme 11beta-HSD1 | Homo sapiens | cortisol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of active site structure of isozyme 11beta-HSD1 from different species, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Homo sapiens |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |