Literature summary for 1.1.1.146 extracted from

Frick, C.; Atanasov, A.G.; Arnold, P.; Ozols, J.; Odermatt, A.
Appropriate function of 11beta-hydroxysteroid dehydrogenase type 1 in the endoplasmic reticulum lumen is dependent on its N-terminal region sharing similar topological determinants with 50-kDa esterase (2004), J. Biol. Chem., 279, 31131-31138.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in HEK293 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E25K	88% of wild type cortisone reduction activity	Homo sapiens
E25K/E26K	20% of wild type cortisone reduction activity	Homo sapiens
E25K/E26Q	45% of wild type cortisone reduction activity	Homo sapiens
E25Q	82% of wild type cortisone reduction activity	Homo sapiens
E25Q/E26Q	57% of wild type cortisone reduction activity	Homo sapiens
E26K	65% of wild type cortisone reduction activity	Homo sapiens
K35S/K36S	no activity	Homo sapiens
K5S/K6S	93% of wild type cortisone reduction activity, no reduction of 7-oxocholesterol in intact HEK-293 cells transfected with 11beta-HSD1	Homo sapiens
K5S/K6S/E25K/E26K	21% of wild type cortisone reduction activity	Homo sapiens
K6S/E25K	86% of wild type cortisone reduction activity	Homo sapiens
K6S/E25K/E26K	31% of wild type cortisone reduction activity	Homo sapiens
K6S/E25K/E26Q	30% of wild type cortisone reduction activity	Homo sapiens
K6S/E25Q/E26Q	43% of wild type cortisone reduction activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00034	-	cortisol	37°C, pH 7.4, oxidation of cortisol by lysates of HEK-293 cells transfected with 11beta-HSD1	Homo sapiens
0.00034	-	cortisone	37°C, pH 7.4, reduction of cortisone by lysates of HEK-293 cells transfected with 11beta-HSD1	Homo sapiens
0.0008	-	cortisol	37°C, pH 7.4, oxidation of cortisol in intact HEK-293 cells transfected with 11beta-HSD1	Homo sapiens
0.0008	-	cortisone	37°C, pH 7.4, reduction of cortisone in intact HEK-293 cells transfected with 11beta-HSD1	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Homo sapiens	5783	-
membrane	membrane bound, luminal orientation of 11beta-HSD1 is essential for efficient oxidation of cortisol	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cortisone + NADPH + H+	Homo sapiens	-	cortisol + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-oxocholesterol + NADPH + H+	luminal orientation of 11beta-HSD1 is essential for the reduction of 7-oxocholesterol	Homo sapiens	7beta-hydroxycholesterol + NADP+	-	?
cortisone + NADPH + H+	-	Homo sapiens	cortisol + NADP+	-	r

Synonyms

Synonyms	Comment	Organism
11beta-HSD1	-	Homo sapiens
11beta-hydroxysteroid dehydrogenase	-	Homo sapiens

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Release 2023.1 (January 2023)