Cloned (Comment) | Organism |
---|---|
expression of 11beta-HSD1 in Pichia pastoris, COS cells and Escherichia coli | Cavia porcellus |
expression of 11beta-HSD1 in Pichia pastoris, COS cells and Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1 | Cavia porcellus | |
0.0003 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1 | Homo sapiens | |
0.0006 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Cavia porcellus | |
0.0008 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Homo sapiens | |
0.002 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris | Cavia porcellus | |
0.002 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris | Homo sapiens | |
0.003 | - |
cortisol | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Cavia porcellus | |
0.003 | - |
cortisol | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Homo sapiens | |
0.008 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1 | Cavia porcellus | |
0.009 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | Cavia porcellus | - |
cortisol + NADP+ | - |
r | |
cortisone + NADPH + H+ | Homo sapiens | - |
cortisol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged-11beta-HSD1 expressed in Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | subcapsular areas of adrenal gland | Cavia porcellus | - |
liver | central vein | Cavia porcellus | - |
liver | central vein | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | - |
Cavia porcellus | cortisol + NADP+ | - |
r | |
cortisone + NADPH + H+ | - |
Homo sapiens | cortisol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Cavia porcellus |
11beta-HSD1 | - |
Homo sapiens |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Cavia porcellus |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
cortisol | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Cavia porcellus | |
0.19 | - |
cortisol | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Homo sapiens | |
0.3 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Cavia porcellus | |
0.4 | - |
cortisone | 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli | Homo sapiens |