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Literature summary for 1.1.1.146 extracted from

  • Blum, A.; Raum, A.; Maser, E.
    Functional characterization of the human 11 beta-hydroxysteroid dehydrogenase 1B (11 beta-HSD 1B) variant (2003), Biochemistry, 42, 4108-4117.
    View publication on PubMed
Search for more literature for 1.1.1.146

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
L150R also lacking the first 30 n-terminal amino acids, gains activity in microsomal fraction after solubilization with Emulgen Homo sapiens
V148R also lacking the first 30 N-terminal amino acids, no activity Homo sapiens
V149E also lacking the first 30 N-terminal amino acids, no activity Homo sapiens
V149R also lacking the first 30 N-terminal amino acids, active and soluble in cytosol, gains activity in microsomal fraction after solubilization with Emulgen Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 cortisol 37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1 Homo sapiens
0.1
-
 cortisone 37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
membrane anchored within the endoplasmic reticulum membrane with one single N-terminal hydrophobic domain Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cortisone + NADPH + H+ Homo sapiens
-
 cortisol + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3 different glycosylation sites Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cortisone + NADPH + H+
-
 Homo sapiens cortisol + NADP+
-
 r

Synonyms

Synonyms Comment Organism
11beta-HSD1
-
 Homo sapiens
11beta-hydroxysteroid dehydrogenase type 1
-
 Homo sapiens