Inhibitors | Comment | Organism | Structure |
---|---|---|---|
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione | i.e. cortisol; poor inhibition of corticosterone oxidation | Rattus sp. | |
11beta,21-dihydroxypregn-4-en-3,20-dione | potent inhibition of cortisol oxidation | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | high- and low Km forms, suggesting 2 isoenzymes, high-Km form represents 90% of total activity in untreated microsomes | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | Rattus sp. | i.e. cortisol | 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | ? | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | Rattus sp. | 10-20fold faster than cortisol oxidation | 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+ | - |
? | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | Rattus sp. | i.e. cortisone | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | Rattus sp. | 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | ? | |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | 10-20fold faster than cortisol oxidation | Rattus sp. | 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+ | - |
? | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | Rattus sp. | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | stimulated enzyme preparation | Rattus sp. |
10 | - |
latent, detergent-treated preparation | Rattus sp. |