Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, catalytic domain | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C272S | ability to form disulfide bonds abolished, but no change in activity or Km | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione | i.e. cortisol | Homo sapiens | |
0.0014 | - |
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione | recombinant catalytic domain | Homo sapiens | |
0.0095 | - |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione | i.e. cortisone | Homo sapiens | |
0.0095 | - |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione | recombinant catalytic domain | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | Homo sapiens | implicated in the pathogenesis of insulin resistance and obesity | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | Homo sapiens | i.e. cortisone | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoenzyme type 1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant catalytic domain | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | implicated in the pathogenesis of insulin resistance and obesity | Homo sapiens | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r | |
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | Homo sapiens | 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | r |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD | 2 isoforms: 11beta-HSD1 and 11beta-HSD2 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |