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Literature summary for 1.1.1.145 extracted from

  • Thomas, J.L.; Boswell, E.L.; Scaccia, L.A.; Pletnev, V.; Umland, T.C.
    Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3b-HSD1) for substrates, coenzymes, and inhibitors relative to human 3b-HSD2 (2005), J. Biol. Chem., 280, 21321-21328.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.145

Cloned(Commentary)

Cloned (Comment) Organism
expression of soluble wild-type and mutant isozyme 3beta-HSD1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens
expression of soluble wild-type and mutant isozyme 3beta-HSD2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Homo sapiens

Protein Variants

Protein Variants Comment Organism
Q105M site-directed mutagenesis of isozyme 1, the mutants show altered kinetics compared to the wild-type isozymes Homo sapiens
Q105M site-directed mutagenesis of isozyme 2, the mutants show altered kinetics compared to the wild-type isozymes Homo sapiens
Q240R site-directed mutagenesis of isozyme 1, the mutant shows altered kinetics compared to the wild-type isozyme Homo sapiens
R240Q site-directed mutagenesis of isozyme 2, the mutant shows altered kinetics compared to the wild-type isozyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
epostane
-
 Homo sapiens
trilostane
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity Homo sapiens
0.0037
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 Homo sapiens
0.0061
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R Homo sapiens
0.025
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M Homo sapiens
0.0256
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R Homo sapiens
0.0327
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q Homo sapiens
0.0341
-
 NAD+ pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 Homo sapiens
0.0372
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q Homo sapiens
0.0388
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M Homo sapiens
0.0405
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M Homo sapiens
0.0473
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 Homo sapiens
0.0614
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M Homo sapiens
0.0863
-
 NAD+ pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14060 3beta-HSD1; isozyme 3beta-HSD1, bifunctional enzyme
-
Homo sapiens P26439 3beta-HSD2; isozyme 3beta-HSD2, bifunctional enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble wild-type and mutant isozyme 3beta-HSD1 from Sf9 insect cells Homo sapiens
recombinant soluble wild-type and mutant isozyme 3beta-HSD2 from Sf9 insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland isozyme 3beta-HSD2 Homo sapiens
-
breast cancer cell isozyme 3beta-HSD1 Homo sapiens
-
gonad isozyme 3beta-HSD2 Homo sapiens
-
placenta isozyme 3beta-HSD1 Homo sapiens
-
prostate tumor cell isozyme 3beta-HSD1 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + NAD+
-
 Homo sapiens ?
-
 r
additional information bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
3beta-HSD1
-
 Homo sapiens
3beta-HSD2
-
 Homo sapiens
type 1 3beta-hydroxysteroid dehydrogenase/isomerase
-
 Homo sapiens
type 2 3beta-hydroxysteroid dehydrogenase/isomerase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R Homo sapiens
0.023
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R Homo sapiens
0.038
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M Homo sapiens
0.055
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 Homo sapiens
0.058
-
 NAD+ pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 Homo sapiens
0.075
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M Homo sapiens
0.113
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M Homo sapiens
0.118
-
 NAD+ pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 Homo sapiens
0.118
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 Homo sapiens
0.145
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M Homo sapiens
0.187
-
 NAD+ pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q Homo sapiens
0.217
-
 dehydroepiandrosterone pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADH
-
 Homo sapiens